Protocols

This section contains recommended methods to be used in conjunction with certain products.
The recommended protocols are contained within sections relating to the distributor from which the product was purchased.
Which Protocol indicates the recommended protocols for each of these products.


Protocol

 

USA
(Vector only)

 

UK / Canada
(Vector only)

 

Rest of the World

Method for ABC Technique using Monoclonal Antibodies on Frozen Sections

 

ABCM-FRO.pdf

 

ABCM-FRO.pdf

 

ABCM-FRO.pdf

Method for ABC Technique using Monoclonal Antibodies on Paraffin Sections

 

ABCM-PAR.pdf

 

ABCM-PAR.pdf

 

ABCM-PAR.pdf

Method for ABC Technique for using Polyclonal Antibodies on Frozen Sections

 

ABCP-FRO.pdf

 

ABCP-FRO.pdf

 

ABCP-FRO.pdf

Method for ABC Technique for using Polyclonal Antibodies on Paraffin Sections

 

ABCP-PAR.pdf

 

ABCP-PAR.pdf

 

ABCP-PAR.pdf

Preparation and use of Zamboni's-fixed Frozen Sections in Immunohistochemistry

 

ZAMB-IHC.pdf

 

ZAMB-IHC.pdf

 

ZAMB-IHC.pdf

Trypsin Digestion of Paraffin sections prior to Immunohistochemistry

 

TRYPSIN.pdf

 

TRYPSIN.pdf

 

TRYPSIN.pdf

High Temperature Antigen Unmasking Technique using Sodium Citrate Buffer for Immunohistochemical Demonstration on Paraffin Sections

 

HTAUT.pdf

 

HTAUT.pdf

 

HTAUT.pdf

High Temperature Antigen Unmasking Technique using EDTA for Immunohistochemical Demonstration on Paraffin Sections

 

HTAUT.pdf

 

HTAUT.pdf

 

HTAUT.pdf

High Temperature Antigen Unmasking Technique using Citrate Buffer in conjunction with the RTU Universal Quick Kits for Immunohistochemical Demonstration on Paraffin Sections

 

HTAUT.pdf

 

HTAUT.pdf

 

HTAUT.pdf

High Temperature Antigen Unmasking Technique using EDTA in conjunction with the RTU Universal Quick Kits for Immunohistochemical Demonstration on Paraffin Sections

 

HTAUT.pdf

 

HTAUT.pdf

 

HTAUT.pdf

Method for the use of Novocastra Antibodies in Western Blotting

 

WEST-MET.pdf

 

WEST-MET.pdf

 

WEST-MET.pdf

Methods for the use of Novocastra Antibodies in Direct Flow Cytometry

 

FLOW_MET.pdf

 

FLOW_MET.pdf

 

FLOW_MET.pdf

Methods for the use of Novocastra Antibodies in Indirect Flow Cytometry

 

IND_FLOW.pdf

 

IND_FLOW.pdf

 

IND_FLOW.pdf

Methods for the use of FITC-Conjugated Antiviral Antibodies in Direct Immunofluorescence

 

DIR_IF.pdf

 

DIR_IF.pdf

 

DIR_IF.pdf

Methods for the use of Antiviral Antibodies in Indirect Immunofluorescence

 

INDIR_IF.pdf

 

INDIR_IF.pdf

 

INDIR_IF.pdf

Methods for using Primer Sets from Novocastra Laboratories

 

PRIMERSE.pdf

 

PRIMERSE.pdf

 

PRIMERSE.pdf

In Situ Hybridisation Method

 

ISHMETH.pdf

 

ISHMETH.pdf

 

ISHMETH.pdf

Method for ABC Technique using Monoclonal Antibodies on Cultured Cells Grown on Glass Cover Slips

 

MONOCULT.pdf

 

MONOCULT.pdf

 

MONOCULT.pdf

Immunohistochemical use of a selection of Monoclonal Antibodies on Frozen Muscle Block Sections

 

MUSCDIS.pdf

 

MUSCDIS.pdf

 

MUSCDIS.pdf

Immunohistochemical use of Monoclonal Antibodies to Dystrophin

 

DYS1_2_3.pdf

 

DYS1_2_3.pdf

 

DYS1_2_3.pdf

Immunohistochemical Detection of Estrogen and Progesterone Receptors on Frozen Sections

 

ER_PGR.pdf

 

ER_PGR.pdf

 

ER_PGR.pdf

Pretreatment required for NCL-BrdU

 

PRE-BRDU.pdf

 

PRE-BRDU.pdf

 

PRE-BRDU.pdf

Flow Cytometry method for the use of the Novocastra Antibody NCL-NB84-FITC

 

NB84_FLO.pdf

 

NB84_FLO.pdf

 

NB84_FLO.pdf

Method for the use of Novocastra PCNA (NCL-PCNA) Antibody: Flow Cytometry

 

PCNA_FLO.pdf

 

PCNA_FLO.pdf

 

PCNA_FLO.pdf

Method for the use of NCL-CRYPTO-FITC Antibody in Direct Immunofluorescence

 

CF-F_DIR.pdf

 

CF-F_DIR.pdf

 

CF-F_DIR.pdf

Method for the use of NCL-GI-340-FITC antibody in Direct Immunofluorescence

 

GI-340_f.pdf

 

GI-340_f.pdf

 

GI-340_Fpdf

Method for the use of NCL-CMVpp65 in Indirect Immunofluorescence

 

CMV_PP65.pdf

 

CMV_PP65.pdf

 

CMV_PP65.pdf

Method for the use of NCL-LPNSG1 in Indirect Immunofluorescence

 

LPNSG1.pdf

 

LPNSG1.pdf

 

LPNSG1.pdf

Method for the use of NCL-P-CAR-344 antibody in Indirect Immunofluorescence

 

PCAR-344.pdf

 

PCAR-344.pdf

 

PCAR-344.pdf

Immunohistochemical Demonstration of Hepatitus C Virus (NS3 Antigen) in Fixed Liver Sections

 

5STEPPUS.pdf

 

5STEPPUK.pdf

 

5STEPPUK.pdf


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