Description
Large scale, multiple tissue expression screening in a dot blot format
• Easily access a large, comprehensive collection of human or mouse tissues
• BD™ Premium Poly A+ RNA provides exceptional quality
• Profile the expression of genes and ESTs over a broad range of tissues and cancer cell lines
• Normalized to selected housekeeping genes for meaningful expression comparisons
BD™ MTE Multiple Tissue Expression Arrays allow you to profile gene expression over a broad range of tissues simultaneously. Each MTE Array is prepared with high quality BD Premium Poly A+ RNA spotted onto a nylon membrane, ensuring the presence of full-length and rare transcripts. Using poly A+ rather than total RNA increases sensitivity while reducing nonspecific background. BD™ MTE Arrays can be used to infer the potential function of a gene of interest or to choose the tissue(s) from which to clone a fulllength cDNA.
With over 70 tissue-specific poly A+ RNAs, the BD MTE Human Array allows you to accurately profile gene expression in one easy hybridization experiment. Screen for the presence and relative abundance of your gene transcript across a broad spectrum of adult and fetal tissues, and cancer cell lines. For distinguishing between different-sized transcripts, we recommend using BD™ MTN Blots.
The BD MTE Mouse Array is spotted with over 20 different mouse poly A+ RNAs made from BD™ Premium RNA. The array allows you to profile gene expression over a wide range of mouse tissues. We normalize the amount of RNA on the blot using several carefully selected housekeeping genes.
Each array is shipped with a positive control probe and BD ExpressHyb™ Hybridization Solution. With proper handling, BD MTE™ Arrays can be stripped and re-probed several times.
What is normalization?
• Normalization is the process where the amount of each poly A+ RNA loaded onto a blot is adjusted based upon the hybridization intensity of a given house-keeping gene (such as b-actin). In this fashion, the hybridization signal is held to a comparable level across the samples. This differs from simply loading equal amounts of poly A+ RNA in each lane.
Why normalize?
• While developmental changes or treatment may affect the expression of some genes, the level of housekeeping gene expression remains fairly constant for most tissues. As such, it can be designated as the reference to which expression of other genes is measured.
• Measurements are consistent, despite the fact that some tissues are more transcriptionally active than others.
Applications
• Obtain semiquantitative transcript expression data from 200¨C100 tissues in a single hybridization experiment
• Quickly determine presence and relative expression levels of a gene across a broad tissue and cell line panel
• Assess cross-species homology using separate BD MTE Blots
Contents
BD™ MTE Human Array Components
BD MTE Human Array 2
Human Ubiquitin cDNA Control Probe
BD ExpressHyb Hybridization Solution
User Manual (PT3307-1)
BD™ MTE Mouse Array Components
BD MTE Mouse Array
Mouse HPRT cDNA Control Probe
BD ExpressHyb Hybridization Solution
User Manual (PT3004-1)
Preparation and Storage
¨C20¡ãC for Array and Control Probes
Room temperature for BD ExpressHyb Hybridization Solution
Images
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| BD MTE™ Arrays reveal accurate gene expression patterns. BD MTE arrays were hybridized with lactoferrin or human ubiquitin cDNA probes overnight with random-primed, radioactively labeled cDNA probes in BD ExpressHyb Hybridization Solution. Arrays were washed with 2X SSC, 1% SDS at 65¡ã;C, and 0.1X SSC, 0.5% SDS at 55¡ã;C, and were exposed to x-ray film at ¨C70¡ã;C overnight. |
