PPase-2B

Product Cat.# Size
PPase-2B V6361 10u

Description

PPase-2B is a heterodimeric enzyme composed of a 19kDa calcium-binding subunit and a catalytic subunit (61kDa) that binds calmodulin. PPase-2B was originally identified based on its calcium- and calmodulin-dependent activity towards phosphorylase kinase and inhibitor-1. PPase-2B is identical to the brain protein calcineurin, which constitutes up to 1% of total brain protein (1). The immunosuppressive drugs FK-506 and cyclosporin A inhibit PPase-2B activity in immune cells, implicating a role for this enzyme in regulation of the immune system. PPase-2B also plays a major role in regulating secretory functions of a variety of cells (1).

PPase-2B is less sensitive to okadaic acid than PPase-2A and PPase-1, requiring micromolar concentrations of okadaic acid for inhibition. It is not inhibited by Inhibitor-1 or Inhibitor-2. Promega's PPase-2B is isolated from bovine brain.

Protocol

Promega Product Information #9PIV636.

Protein Phosphatase-2B (PPase-2B) is a heterodimeric enzyme composed of a 19kDa calcium-binding subunit and a catalytic subunit (61kDa) that binds calmodulin. PPase-2B was originally identified based on its calcium- and calmodulin-dependent activity towards phosphorylase kinase and inhibitor-1. PPase-2B is identical to the brain protein calcineurin, which constitutes up to 1% of total brain protein. The immunosuppressive drugs FK-506 and cyclosporin A inhibit PPase-2B activity in immune cells, implicating a role for this enzyme in regulation of the immune system. PPase-2B also plays a major role in regulating secretory functions of a variety of cells.

PPase-2B is less sensitive to okadaic acid than PPase-2A and PPase-1, requiring micromolar concentrations of okadaic acid for inhibition. It is not inhibited by Inhibitor-1 or Inhibitor-2. Promega's PPase-2B is isolated from bovine brain.

Storage Conditions

Store at C70C.

Storage Buffer

50mM Tris-HCl (pH 7.0), 50% glycerol.

Unit Definition

One unit is the amount of PPase-2B required to release one nmol of phosphate per minute at 30C, using p-nitrophenylphosphate (PNPP) as a substrate.

Component Listing for PPase-2B

Component Listing for V6361
1 x 10u PPase-2B