Cat. No.	Host Species	Clone Isotype	Format	Applications	Suggested Dilution*	Unit
T61544R	Rabbit	N/A	Purified	IHC - frozen Dot-blot ELISA	1:10 - 1:20 n/a n/a	0.2 mg
T40251R x-reacts with rat	Rabbit	N/A	Purified	IHC - frozen IFA ELISA RIA	1:40 - 1:160 n/a n/a n/a	0.5 mg
M61403M	Mouse	1042 IgG2b	Diluted Ascites	IHC - frozen or paraffin**	1:10 - 1:20	1 ml
M61006M x-reacts with dog	Mouse	MC4-HA IgM	Purified	IHC - frozen or paraffin** ELISA	1:10 - 1:20 n/a	0.2 mg
T61550R	Rabbit	N/A	Affinity Purified	IHC - frozen Dot-blot ELISA	1:10 - 1:20 n/a n/a	1 ml
T59106R	Rabbit	N/A	Affinity Purified	Immunoblot ELISA IHC - frozen	1:5000 - 1:10,000 1:4000 - 1:8000 1:50 - 1:200	0.5 mg

* Since applications vary, these dilutions are suggestions. Each laboratory should determine the optimum dilution for use in its particular application. ** Proteolytic pretreatment with pepsin is recommended. Tissues should be fixed with neutral formalin. Note: Some collagen samples can be contaminated with other collagens. When purified collagen is used in an application, the purity of the collagen sample should be verified by SDS-PAGE to minimize the risk of false positives.

Human Collagen Type IV Antigen

Cat. No.	Source	Purity	Applications	Unit
A33125H	Placental villi	>90% by SDS-PAGE analysis	Standards Antigen for antibody production Coating material for cell culture studies Formation of collagen gels	0.5 mg

FOR RESEARCH USE ONLY.   NOT FOR USE IN DIAGNOSTIC PROCEDURES.
Document Revision   B 050701