|
Cat.
No. |
Host Species |
Clone Isotype |
Format |
Applications |
Suggested Dilution* |
|
Unit |
|
Mouse Ascites |
9001 IgG1, k |
Purified |
ELISA IHC Immunoblot (non-
reducing conditions) |
(1:50
to 1:500 depending on technique used) |
|
1 ml |
|
|
x-react
with most mammalian |
Rabbit |
|
Affinity Purified |
ELISA IHC frozen Immunobot |
1:4000
– 1:8000 1:50
– 1:200 1:5000
– 1:10,000 |
|
0.5 mg |
|
Mouse Ascites |
9001 IgG1, k |
Ascites |
ELISA IHC Immunoblot (non-
reducing conditions) IFA |
(1:50
to 1:500 depending on technique used) |
|
1 ml |
|
|
Rabbit |
|
Neat |
ELISA IHC IFA Immunoblot |
(1:100
to 1:3000 depending on technique used) |
|
1 ml |
|
|
Rabbit |
|
Purifed |
ELISA IHC IFA Immunoblot |
(1:100
to 1:3000 depending on technique used) |
|
1 ml |
*Since
applications vary, these are just suggested dilutions.
Each laboratory should modify dilutions to suit their procedures and
needs.
Note:
Some collagen samples can be contaminated with other collagens.
When purified collagen is used in an application the purity of the
collagen sample should be verified by SDS-Page to minimize the risk of false
positives.
|
Cat.
No. |
Source
|
Purity
|
Applications |
|
Unit |
|
Human placental villi |
>90% by SDS-PAGE analysis |
-Standards -Antigen for antibody production -Coating material for cell culture studies -Formation of collagen gels |
|
0.5 mg |