ELISA Plate Coating Stabilizer and Blocking Buffer


BIODESIGN's Coating Stabilizer and Blocking Buffer is a reagent specifically formulated to improve the stability and function of solid phase proteins (antigens, antibodies, etc.). In comparison to the performance of competitor products, this technology has been shown to both greatly enhance the stability of labile antigens (e.g. the nuclear la antigen) as well as the specific signal in solid phase immunoassay formats. The increased signal is a consequence of the reagent's stabilizing effect on the tertiary structure of proteins on the solid phase. Improved maintenance of tertiary structure results in optimal antigenic function because there is less denatured folding of the protein to mask antigenic regions.

Quality Control

For QC clearance, each lot of stabilizer must pass a rigorous performance test. The test is initiated by treating a labile antigen coated on the surface of microtiter plate wells with each new lot of stabilizer. Treated plates are then dried, sealed in mylar bags along with desiccant and subjected to elevated temperatures. After being stressed for a prescribed period, the solid phase is evaluated for antigenic activity, using an ELISA format, to verify product performance is within specification (significantly enhanced stability of solid phase antigen treated with our coating stabilizer relative to a control).

Product Performance

In accelerated stability studies of eighteen different antigens held at 37C, our Coating Stabilizer and Blocking Buffer was shown to be far superior to the leading competitor in maintaining antigenic function (see attached on back side of this sheet).

Product Feature End User Benefit
Greatly increases the stability of solid phase proteins. Increased product shelf life and enhanced product performance.

Increases stability of tertiary structure of proteins and antigenic regions. Increased immunoassay signal / unit mass of solid phase antigen. Increased antigen use dilution.

Filtered through 0.2mm filter and fortified with a non-mercury and azide - free preservative.

No sterility, shipping or waste disposal problems due to bioburden or preservative.
Formulated as a stand alone coating stabilizer and blocking reagent or can be diluted up to 1:2 in your current diluent or blocking solution.

Ease of use. Economical and accommodating.



Recommended Protocol for Stabilizing and Blocking Immobilized Proteins

1. After coating the surface with protein/antigen, wash once to remove excess and weakly adsorbed protein.

2. Before the protein begins to dry, coat the surface with the Coating Stabilizer and Blocking Buffer to completely cover the bound material. Allow to incubate at room temperature for 15 to 60 minutes.

3. Aspirate or drain the excess stabilizer from the surface. Do not wash the surface.

4. Dry the protein, preferably under vacuum. Recommended drying times are as follows:

a. Two hours under vacuum (< 100 micron)
or
b. Overnight in a humidity controlled chamber that registers <15% humidity.

5. Package the bound antigen/protein in a sealed airtight container with desiccant. The product is now stabilized for long-term storage at 2 to 8C.



Available: Catalog # J16430D in 125 mL, 500 mL, 1000 mL packaging as well as bulk packaging.

For Research Use Only. Not For Use In Diagnostic Procedures.

Comparison Showing the Relative Effectiveness of the BIODESIGN Coating Stabilizer and Blocking Buffer