For QC clearance, each lot of stabilizer must pass a rigorous performance test. The test is initiated by treating a labile antigen coated on the surface of microtiter plate wells with each new lot of stabilizer. Treated plates are then dried, sealed in mylar bags along with desiccant and subjected to elevated temperatures. After being stressed for a prescribed period, the solid phase is evaluated for antigenic activity, using an ELISA format, to verify product performance is within specification (significantly enhanced stability of solid phase antigen treated with our coating stabilizer relative to a control).
In accelerated stability studies of eighteen different antigens held at 37°C, our Coating Stabilizer and Blocking Buffer was shown to be far superior to the leading competitor in maintaining antigenic function (see attached on back side of this sheet).
Recommended Protocol for Stabilizing and Blocking Immobilized Proteins
1. After coating the surface with protein/antigen, wash once to remove excess and weakly adsorbed protein.
2. Before the protein begins to dry, coat the surface with the Coating Stabilizer and Blocking Buffer to completely cover the bound material. Allow to incubate at room temperature for 15 to 60 minutes.
3. Aspirate or drain the excess stabilizer from the surface. Do not wash the surface.
4. Dry the protein, preferably under vacuum. Recommended drying times are as follows:
a. Two hours under vacuum (< 100 micron)
orb. Overnight in a humidity controlled chamber that registers <15% humidity.
5. Package the bound antigen/protein in a sealed airtight container with desiccant. The product is now stabilized for long-term storage at 2 to 8°C.
Available: Catalog # J16430D in 125 mL, 500 mL, 1000 mL packaging as well as bulk packaging.
For Research Use Only. Not For Use In Diagnostic Procedures.
Comparison Showing the Relative Effectiveness of the BIODESIGN Coating Stabilizer and Blocking Buffer