HRP Conjugate Stabilizer

BIODESIGN's new HRP Stabilizer is formulated specifically for Horseradish Peroxidase conjugates. This formulation insures consistently high levels of activity for both the enzyme and the antibody following long-term storage at final use dilution.

Quality Control

For QC clearance, each lot of HRP stabilizer must pass a rigorous performance test. The test is performed by formulating an HRP conjugate at use dilution in each new lot of stabilizer and then stressing the formulation at elevated temperatures. After a prescribed incubation period, the conjugate is evaluated for functional activity in an ELISA format to verify product performance is within specification. Specifically, HRP conjugates formulated in our HRP conjugate stabilizer must show significantly enhanced stability relative to a control.

Product Performance

In a comparative accelerated stability study, our HRP conjugate stabilizer was shown to be far superior to common conjugate diluents and to a major competitor's product in stabilizing HRP conjugates against degradation at elevated temperatures. An equally important observation was the finding that our HRP conjugate stabilizer out performed competitor formulations with improved signal to noise ratios in ELISA formats. The following graph compares the relative stability of a HRP anti-human GAM conjugate stressed at 50°C over a four week period when formulated in our HRP Conjugate Stabilizer and two other conjugate diluents respectively:

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Product Feature End User Benefit
Improves signal-to-noise ratios. Increased immunoassay sensitivity.

Cleaner arrays.
Greatly increases conjugate stability. Increased shelf life

Improved resistance to variations in shipping and storage temperatures.

Improved day to day assay precision.
Filtered through 0.2mm filter and fortified with a non-mercury and azide - free preservative.

 No sterility, shipping or waste disposal problems due to bioburden or preservative.
Formulated to be diluted up to 1:2 to achieve conjugate use dilution.

 Ease of use - dilute to use dilution and your conjugate is ready to vial.
Economical.

 The 1:2 final dilution significantly reduces costs.



Recommended Protocol for Preparing HRP Conjugates in the HRP Conjugate Stabilizer

1. The new HRP Conjugate Stabilizer is supplied ready to use, but also can be diluted up to 1:1 (final use dilution of stabilizer) in a saline solution (0.9% NaCl) to give greater product economy without compromising performance. Add the HRP conjugate to this stabilizer solution to achieve the desired dilution for your application. HEPES and MOPS buffers are highly compatible with the stabilizing solution, but only very small amounts of phosphate buffered solutions may be added.

2. Accelerated stability studies can be performed for several days at 50-55°C to insure efficacy of the stabilizing solution with your particular applications.

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Catalog # J16200D available in 50mL, 125mL, 1000mL packaging as well as bulk packaging.

For Research Use Only. Not For Use In Diagnostic Procedures.