Further information:
QIAGEN Plasmid Purification System

QIAGEN Plasmid Kits

For purification of up to 10 mg ultrapure plasmid or cosmid DNA

Classic QIAGEN Plasmid Kits use gravity-flow anion-exchange tips for efficient purification of plasmid DNA (for principle, see page 199).

Features and benefits

Purity equivalent to that obtained by 2x CsCl-gradient centrifugation
Reproducible yields of ultrapure DNA
No ethidium bromide, no phenol, no chloroform, no CsCl
Cost effective mini,* midi, maxi, mega, and giga preparations

QIAGEN Plasmid Kit Options
	Expected yield†	Column capacity	Culture volume‡
QIAGEN Plasmid Mini Kit*	Up to 20 µg	20 µg	3 –10 ml
QIAGEN Plasmid Midi Kit	Up to 100 µg	100 µg	25 –100 ml
QIAGEN Plasmid Maxi Kit	Up to 500 µg	500 µg	100 ml –500 ml
QIAGEN Plasmid Mega Kit	Up to 2.5 mg	2.5 mg	500 ml – 2.5 liters
QIAGEN Plasmid Giga Kit	Up to 10 mg	10 mg	2.5 – 5 liters

* Note: For other miniprep spin and vacuum formats, See Plasmid Miniprep Kit Selection guide.
† Actual yields depend on plasmid copy number, size of insert, host strain, culture medium, and culture volume.
‡ Culture volumes depend on plasmid copy number, size of insert, host strain, and culture medium.

Procedure

With QIAGEN Plasmid Kits, bacterial lysates are cleared by centrifugation and the cleared lysate is applied to the QIAGEN-tip for plasmid DNA purification. Eluted ultrapure DNA is recovered by isopropanol precipitation and centrifugation.

Applications

Plasmid DNA purified with QIAGEN Plasmid Kits is ideal for use in applications such as transfection (see figure "Transfection Efficiency vs Plasmid Purification Method"), cloning, manual or automated sequencing, including capillary sequencing, and in vitro transcription.

操作手册: QIAGEN Plasmid Purification Handbook 9/00 (PDF version, 389 KB)

Ordering Information
Product	Contents	Cat. No.
QIAGEN Plasmid Mini Kit (25)	25 QIAGEN-tip 20, Reagents, Buffers	12123

QIAGEN Plasmid Mini Kit (100)	100 QIAGEN-tip 20, Reagents, Buffers	12125

QIAGEN Plasmid Midi Kit (25)	25 QIAGEN-tip 100, Reagents, Buffers	12143

QIAGEN Plasmid Midi Kit (100)	100 QIAGEN-tip 100, Reagents, Buffers	12145

QIAGEN Plasmid Maxi Kit (10)	10 QIAGEN-tip 500, Reagents, Buffers	12162

QIAGEN Plasmid Maxi Kit (25)	25 QIAGEN-tip 500, Reagents, Buffers	12163

QIAGEN Plasmid Maxi Kit (100)	100 QIAGEN-tip 500, Reagents, Buffers	12165

QIAGEN Plasmid Mega Kit (5)	5 QIAGEN-tip 2500, Reagents, Buffers	12181

QIAGEN Plasmid Mega Kit (25)	25 QIAGEN-tip 2500, Reagents, Buffers	12183

QIAGEN Plasmid Giga Kit (5)	5 QIAGEN-tip 10000, Reagents, Buffers	12191