Further information:
QIAGEN Plasmid Purification System

QIAfilter Plasmid Kits

For fast purification of up to 10 mg ultrapure plasmid or cosmid DNA

Features and benefits

Up to 10 mg of ultrapure plasmid DNA
Cleared lysates from up to 2.5 liters of LB culture without centrifugation
Reduced plasmid purification time
Fast, easy, and convenient handling for small to large culture volumes

QIAfilter Plasmid Kit Options
	Expected yield*	Column capacity	Culture volume†	QIAfilter format
QIAfilter Plasmid Midi Kit	Up to 100 µg	100 µg	25–50 ml	Syringe-format Midi Cartridge
QIAfilter Plasmid Maxi Kit	Up to 500 µg	500 µg	100–250 ml	Syringe-format Maxi Cartridge
QIAfilter Plasmid Mega Kit	Up to 2.5 mg	2.5 mg	500 ml–2.5 liters	Vacuum-operated Mega-Giga Cartridge
QIAfilter Plasmid Giga Kit‡	Up to 10 mg	10 mg	2.5 liters	Vacuum-operated Mega-Giga Cartridge

* Actual yields depend on plasmid copy number, size of insert, host strain, culture medium, and culture volume.
† Culture volumes depend on plasmid copy number, size of insert, host strain, and culture medium.
‡ For purification of low-copy plasmids and cosmids, QIAfilter Plasmid Mega Kits are a better choice than QIAfilter Plasmid Giga Kits, due to the large culture volumes required and the limited capacity of the QIAfilter Mega-Giga Cartridge.

QIAfilter principle

QIAfilter Cartridges, provided in QIAfilter, HiSpeed, and EndoFree Plasmid Kits, are special filter units designed to replace centrifugation following alkaline lysis of bacterial cells. QIAfilter Cartridges completely remove SDS precipitates and clear bacterial lysates in a fraction of the time needed for centrifugation, reducing plasmid-purification time by up to 1 hour.

QIAfilter Midi and Maxi Cartridges have a syringe-format (see figure "QIAfilter Midi Cartridge") and lysates are cleared in a matter of seconds by pushing the liquid through the filter. QIAfilter Mega-Giga Cartridges operate with house vacuum to efficiently clear even large volumes of bacterial lysate with minimal effort (see figure "QIAfilter Mega-Giga Cartridge").

Procedure

Neutralized bacterial lysates are incubated directly in the QIAfilter Cartridge and cleared in seconds by filtration. The filtrate is then applied directly to a QIAGEN-tip for plasmid DNA purification (see flowchart "Comparison of QIAGEN Plasmid Kit Procedure").

Applications

DNA obtained with QIAfilter Plasmid Kits provides excellent results in all applications, from cloning to transfection and automated fluorescent sequencing, including capillary sequencing.

QIAfilter Midi Cartridge		QIAfilter Mega-Giga Cartridge

The syringe-format QIAfilter Midi Cartridge in use.		The vacuum-operated QIAfilter Mega- Giga Cartridge in use. Please note that the bottle is not included in the kits.

操作手册: QIAGEN Plasmid Purification Handbook 9/00 (PDF version, 389 KB)

Ordering Information
Product	Contents	Cat. No.
QIAfilter Plasmid Midi Kit (25)	25 QIAGEN-tip 100, Reagents, Buffers, 25 QIAfilter Midi Cartridges	12243

QIAfilter Plasmid Midi Kit (100)	100 QIAGEN-tip 100, Reagents, Buffers, 100 QIAfilter Midi Cartridges	12245

QIAfilter Plasmid Maxi Kit (10)	10 QIAGEN-tip 500, Reagents, Buffers, 10 QIAfilter Maxi Cartridges	12262

QIAfilter Plasmid Maxi Kit (25)	25 QIAGEN-tip 500, Reagents, Buffers, 25 QIAfilter Maxi Cartridges	12263

QIAfilter Plasmid Mega Kit (5)	5 QIAGEN-tip 2500, Reagents, Buffers, 5 QIAfilter Mega-Giga Cartridges	12281

QIAfilter Plasmid Giga Kit (5)	5 QIAGEN-tip 10000, Reagents, Buffers, 5 QIAfilter Mega-Giga Cartridges	12291

QIAfilter Midi Cartridges (25)	25 QIAfilter Midi Cartridges	19743

QIAfilter Maxi Cartridges (25)	25 QIAfilter Maxi Cartridges	19763

QIAfilter Mega-Giga Cartridges (5)	5 QIAfilter Mega-Giga Cartridges, Buffer FWB	19781

QIArack	1 rack for 12 x QIAGEN-tip 20, 8 x QIAGEN-tip 100, 6 x QIAGEN-tip 500 or HiSpeed Midi Tips, 4 x QIAGEN-tip 2500 or HiSpeed Maxi Tips, or 10 x QIAfilter Midi or Maxi Cartridges	19015

* For purification of low-copy plasmids and cosmids, QIAfilter Plasmid Mega Kits are a better choice than QIAfilter Plasmid Giga Kits, due to the large culture volumes required and the limited capacity of the QIAfilter Mega-Giga Cartridge.