QIAGEN Lambda System

The QIAGEN Lambda System is designed for preparation of 10¨C300 µg ultrapure lambda DNA for use in all downstream applications.

QIAGEN Lambda Kit Options
  Expected yield* Recommended
volume of
liquid lysate*		 Recommended number of plates*
 
  90 mm plates 140 mm plates
QIAGEN Lambda Mini Kit Up to 12 µg 10 ml 1 plate
(6 ml lysate)		 0.5 plates
(6 ml lysate)
QIAGEN Lambda Midi Kit Up to 60 µg 50 ml 4 plates
(24 ml lysate)		 2 plates
(24 ml lysate)
QIAGEN Lambda Maxi Kit Up to 300 µg 250 ml 20 plates
(120 ml lysate)		 10 plates
(120 ml lysate)

* Optimal starting volumes and actual yields depend on the phage titer. Plate lysates typically contain 1010 to 1011 pfu/ml. Lysates from liquid cultures generally have lower and more variable phage titers of 5 x 109 to 3 x 1010 pfu/ml. QIAGEN-tips 20, 100, and 500 have maximum lambda-DNA-binding capacities of 12, 60, and 300 µg, respectively. Actual yields will depend on phage titer and lysate volume.

Features and benefits

Principle and Procedure

The QIAGEN Lambda System utilizes the QIAGEN-tips containing the unique QIAGEN Anion-Exchange Resin. Isolated phage DNA is free of protein and RNA ¡ª without the use of CsCl, phenol, or chloroform. Up to 300 µg of ultrapure phage DNA can be purified in under 4 hours. Phages are first isolated from liquid or plate lysates using an optimized PEG precipitation step (see flowchart "QIAGEN Lambda Procedure"). The phage particles are then lysed, and the phage DNA is selectively bound under low-salt and pH conditions. Impurities are further removed by a medium-salt wash. In the final step, pure lambda DNA is eluted in high-salt buffer and concentrated and desalted by isopropanol precipitation.

Applications

Using this system DNA yields are highly reproducible and DNA is suitable for automated or manual sequencing, PCR, and in vitro packaging.

 

For preparation of 10¨C300 µg ultrapure lambda DNA

²Ù×÷ÊÖ²á:QIAGEN Lambda Handbook 02/99 (PDF version, 112 KB)

Ordering Information
Product Contents Cat. No.
QIAGEN Lambda Mini Kit (25) 25 QIAGEN-tip 20, Reagents, Buffers 12523
QIAGEN Lambda Midi Kit (25) 25 QIAGEN-tip 100, Reagents, Buffers 12543
QIAGEN Lambda Maxi Kit (10) 10 QIAGEN-tip 500, Reagents, Buffers 12562