QIAGEN RNA/DNA Kits are designed for isolation of total RNA, parallel isolation of RNA and genomic DNA from the same sample, and isolation of low-molecular-weight RNA species such as tRNA and 5S rRNA.
Parallel Isolation of RNA and DNA
Parallel isolation of total RNA and genomic DNA using the QIAGEN RNA/DNA Kit. A Formaldehyde agarose gel (1.2%) of total RNA and B TBE agarose gel (0.8%) of genomic DNA isolated from 1 x 107 chicken liver cells (C) or 50 mg mouse liver tissue (T) using QIAGEN-tip 100. Yields from (C) and (T) were 135 and 210 µg RNA, and 62 and 79 µg DNA, respectively. Total RNA (12.5 µg) and genomic DNA, undigested (2 µg) or digested (4 µg) as indicated, were loaded. RM: 0.24¨C9.5 kb RNA markers; DM: lambda¨CHindIII.
QIAGEN RNA/DNA Kits allow isolation of total RNA, parallel isolation of RNA and genomic DNA from the same sample, and isolation of low-molecular-weight (LMW) RNA molecules* such as tRNA and 5S rRNA. The kits utilize the unique binding properties of QIAGEN Resin to selectively purify different classes of nucleic acids by varying the wash or elution conditions (see figure "Parallel Isolation of RNA and DNA"). QIAGEN RNA/DNA Kits allow nucleic acid isolation even from small amounts of animal cells or tissues, yeast,† or bacteria.‡ QIAGEN RNA/DNA Kits can also be used to purify large amounts of in vitro transcripts or to clean up RNA after enzymatic reactions.
* Additional buffer required. The
buffer composition is provided in the protocols.
† Lyticase, zymolase, or glass beads (required for yeast samples) are not
provided.
‡ Lysozyme (required for bacterial samples) is not provided.
²Ù×÷ÊÖ²á: QIAGEN RNA/DNA Handbook (PDF version, 374 KB)