QIAGEN Taq DNA Polymerase

QIAGEN Taq DNA Polymerase products provide robust PCR performance in a wide range of PCR applications without the need for time-consuming optimization.

Features and benefits

• QIAGEN PCR Buffer for minimal PCR optimization
• Q-Solution, an innovative PCR additive, for amplification of templates that are GC rich or that have extensive secondary structure
• Choice of formats for convenience and ease of handling

Product Format Options
Components	Taq DNA Polymerase	Taq PCR Core Kit	Taq PCR Master Mix Kit (premixed solution)*
Taq DNA Polymerase
PCR Buffer†
Q-Solution			—
dNTP Mix‡	—
MgCl2 solution
Distilled water	—	—

* Taq PCR Master Mix is a premixed solution containing Taq DNA Polymerase, PCR Buffer, and dNTPs. The solution provides a final concentration of 1.5 mM MgCl2 and 200 µM each dNTP.
† Provides a final concentration of 1.5 mM MgCl2
‡ Provides a final concentration of 200 µM each dNTP

Principle

QIAGEN PCR Buffer has been developed to save time and effort by reducing the need for PCR optimization. QIAGEN PCR Buffer contains both KCl and (NH4)2SO4 providing important advantages over conventional buffers, such as a wider temperature window for specific annealing and a greater tolerance to variable Mg2+ concentration (see figures: "Wide Annealing-Temperature Window and Tolerance to Variable Mg2+ Concentration").

Q-Solution facilitates amplification of difficult templates by modifying the melting behavior of DNA. Use of this unique reagent will often enable or improve suboptimal PCR (see figure "Amplification of Difficult Templates with Q-Solution"). Unlike DMSO and other PCR additives, Q-Solution is used at a defined working concentration with any primer–template system and is not toxic.

QIAGEN Taq DNA Polymerase is a high-quality recombinant enzyme that is suitable for general and specialized PCR applications (see figures: "Tolerance of Different Primer Tm Values" and "Specific Amplification of Long PCR Products"). Every lot of QIAGEN Taq DNA Polymerase is subjected to a comprehensive range of quality control tests, including a stringent PCR specificity and reproducibility assay in which low-copy targets are amplified from human genomic DNA (see figure "Lot-to-Lot Reproducibility" and table "QIAGEN Taq DNA Polymerase specifications").

Amplification of Difficult Templates with Q-Solution

Tolerance of Different Primer Tm Values

Taq PCR Master Mix provides QIAGEN Taq DNA Polymerase in a premixed format. This readyto-use solution includes Taq DNA Polymerase, PCR Buffer, MgCl2, and ultrapure dNTPs at optimized concentrations. Only primers and template DNA are added to prepare the final PCR. Taq PCR Master Mix contributes to highly reproducible PCR by reducing pipetting errors and miscalculation (see figure "Reproducible PCR with Master Mix"). Taq PCR Master Mix can be stored at 2–8°C allowing even faster PCR setup by eliminating thawing time.

Reproducible PCR with Master Mix

Specific Amplification of Long PCR Products

Lot-to-Lot Reproducibility

QIAGEN Taq DNA Polymerase specifications

Concentration: 5 units/µl Recombinant enzyme: Yes Substrate analogs: dNTP, ddNTP, dUTP,   biotin-11-dUTP,   DIG-11-dUTP,   fluorescent-dNTP/ddNTP Extension rate: 2–4 kb/min at 72°C Half-life: 10 min at 97°C   60 min at 94°C

Amplification efficiency: ≥105 fold 5'3' exonuclease activity: Yes Extra A addition: Yes 3'5' exonuclease activity: No Contaminating nucleases: No Contaminating RNases: No Contaminating proteases: No Self-priming activity: No

Applications

QIAGEN Taq DNA Polymerase is used for standard and specialized applications including:

• General PCR
• RT-PCR
• Differential display
• Multiplex PCR
• PCR-based DNA fingerprinting (VNTR, STR, and RAPD)

操作手册:Taq PCR Handbook 03/02 (PDF version, 154 KB)

Taq PCR Master Mix Kit

Premixed solution for convenient PCR setup

Component Included Concentration Taq PCR Master Mix 2x premixed solution* Distilled water —

* Taq PCR Master Mix is a premixed solution containing Taq DNA Polymerase, PCR Buffer, and dNTPs. The solution provides a final concentration of 1.5 mM MgCl2 and 200 µM each dNTP.