Further information:
QuantiTect PCR and RT-PCR System

QuantiTect SYBR Green RT-PCR Kit

For quantitative, real-time one-step RT-PCR

Component
Included
Concentration
QuantiTect SYBR Green RT-PCR Master Mix
2x premixed solution*
QuantiTect RT Mix
Premixed solution†
RNase-free water
！
* QuantiTect SYBR Green RT-PCR Master Mix is a premixed solution containing HotStarTaq DNA Polymerase, QuantiTect SYBR Green RT-PCR Buffer, dNTPs (including dUTP for optional uracil-N-glycosylase treatment), SYBR Green I, and ROX (passive reference dye).
† QuantiTect RT Mix contains an optimized mixture of Omniscript and Sensiscript Reverse Transcriptases.

In one-step quantification of RNA using the QuantiTect SYBR Green RT-PCR Kit, sensitivity is enhanced by combining HotStarTaq DNA Polymerase with a unique blend of Sensiscript and Omniscript Reverse Transcriptases. Sensiscript RT is designed for use with very small amounts of RNA, while Omniscript RT provides highly efficient reverse transcription with RNA amounts greater than 50 ng. The combination of both of these high-affinity reverse transcriptases ensures highly efficient, sensitive, and reproducible reverse transcription over a wide range of template amounts (see figure "High Reproducibility and Sensitivity of the QuantiTect SYBR Green RT-PCR Kit").

High Reproducibility and Sensitivity of the QuantiTect SYBR Green RT-PCR Kit

　

One-step RT-PCR of a cyclophilin fragment from 100 ng, 1 ng, and 10 pg of total HeLa RNA. Twelve samples were amplified in parallel from each template amount. Coefficient of variation (CV) of the CT value was below 1.1% for all 3 template amounts. Reactions were carried out on the ABI PRISM 7700 Detection System.

荷恬返過:QuantiTect SYBR Green RT-PCR Handbook 03/02 (PDF version, 192 KB)　

Ordering Information
Product Contents Cat. No.
QuantiTect SYBR Green RT-PCR Kit (200) For 200 x 50 µl reactions: 3 x 1.7 ml QuantiTect SYBR Green RT-PCR Master Mix,* 100 µl QuantiTect RT Mix, 2 x 2.0 ml RNase-free water 204243

* Contains 5 mM MgCl2