Further information:
MinElute and QIAquick DNA
Cleanup Systems
QIAquick PCR Purification Kit
For cleanup of DNA from PCR or other
enzymatic reactions
| Application: |
PCR cleanup |
 |
| Format: |
Spin columns |
| Throughput: |
1¨C24 samples |
| DNA size
range: |
100 bp ¨C 10 kb |
| Equipment
required: |
Microcentrifuge or vacuum manifold |
Features and benefits
- Purified ds or ssDNA in 5 minutes from PCR
amplification
- 90¨C95% recovery of fragments (100 bp ¨C 10 kb)
- >99.5% primer removal (Figure 11.4)
- Separation of >100 bp product from 40mer primer
- No removal of mineral oil necessary
- Elution in as little as 30 µl Tris buffer or
water
Complete
Primer Removal after PCR
 |
Analysis of PCR products before (b)
and after (a) purification
with the QIAquick PCR Purification Kit. Samples were analyzed
on a 1% TAE agarose gel. M: markers.
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|
²Ù×÷ÊÖ²á: QIAquick
Spin Handbook 03/01 (PDF version, 643 KB)
| Ordering
Information |
| Product |
Contents |
Cat. No. |
| QIAquick
PCR Purification Kit (50) |
For
purification of 50 PCR reactions: 50 QIAquick Spin Columns,
Buffers, Collection Tubes (2 ml) |
28104 |
|
| QIAquick
PCR Purification Kit (250) |
For
purification of 250 PCR reactions: 250 QIAquick Spin Columns,
Buffers, Collection Tubes (2 ml) |
28106 |
|
| Buffer
PB (500 ml) |
500 ml
Binding Buffer |
19066 |
|
| Buffer
PE (concentrate, 100 ml) |
100 ml Wash
Buffer (5x concentrate for 500 ml buffer) |
19065 |
|