Further information:
MinElute and QIAquick DNA
Cleanup Systems
For DNA cleanup from enzymatic reactions
| Application: | Cleanup of enzymatic reactions | ![]() |
| Format: | Spin columns | |
| Throughput: | 1¨C24 samples | |
| DNA size range: | 70 bp ¨C 4 kb* | |
| Equipment required: | Microcentrifuge or vacuum manifold | |
* DNA fragments larger than 4 kb should be purified using the QIAquick System
Examples of enzymes that are completely removed by the MinElute Reaction Cleanup Kit
| Protein | Molecular weight per enzyme subunit (kDa) |
|
|
|
| DNA Polymerase I | 109 |
| Klenow fragment | 62 |
| Calf intestinal alkaline phosphatase | 69 |
| T4 DNA ligase | 55 |
| T4 Polynucleotide kinase | 35 |
| Terminal transferase | 32 |
| DNase I | 31 |
| Restriction enzymes | Varies |
²Ù×÷ÊÖ²á: MinElute Handbook 04/01 (PDF version, 679 KB)