Further information:
MinElute and QIAquick DNA Cleanup Systems

QIAquick Nucleotide Removal Kit

For oligonucleotide and DNA cleanup from enzymatic reactions

Application:	Nucleotide removal*
Format:	Spin columns
Throughput:	1–24 samples
DNA size range:	17mer – 10 kb
Equipment required:	Microcentrifuge or vacuum manifold

* For sample volumes smaller than 50 µl the DyeEx Spin kit can also be used
† Vacuum processing is not recommended for radioactive samples.

Purified ds or ssDNA in 5 minutes from enzymatic reactions such as labeling, dephosphorylation, restriction, and tailing
60–80% recovery of oligonucleotides (17–40mers)
80–95% recovery of fragments (40 bp – 10 kb)
99% removal of unincorporated nucleotides (see figure "Complete Removal of Nucleotides from Labeled Oligos")
Elution in as little as 30 µl Tris buffer or water

Complete Removal of Nucleotides from Labeled Oligos

Polyacrylamide gel analysis of radioactive labeling reactions
before (b) and after (a) purification using the QIAquick
Nucleotide Removal Kit.

Ordering Information
Product	Contents	Cat. No.
QIAquick Nucleotide Removal Kit (50)	50 QIAquick Spin Columns, Buffers, Collection Tubes (2 ml)	28304

QIAquick Nucleotide Removal Kit (250)	250 QIAquick Spin Columns, Buffers, Collection Tubes (2 ml)	28306

Buffer PN (500 ml)	500 ml Binding Buffer	19071

Buffer PE (concentrate, 100 ml)	100 ml Wash Buffer (5x concentrate for 500 ml buffer)	19065