Further information:
MinElute and QIAquick DNA Cleanup Systems

MinElute Gel Extraction Kit

For gel extraction of DNA fragments in low elution volumes

Application:	Gel extraction
Format:	Spin columns
Throughput:	1–24 samples
DNA size range:	70 bp – 4 kb*
Equipment required:	Microcentrifuge or vacuum manifold

* DNA fragments from 4 kb–10 kb should be purified using the QIAquick Gel Extraction Kit and DNA fragments smaller
than 70 bp or larger than 10 kb should be extracted with the QIAEX II Gel Extraction System.

Features and benefits

• Elution with just 10 µl Tris buffer or water
• Highly concentrated purified DNA
• DNA fragments extracted in 18 minutes
• 80% recovery of fragments (70 bp – 4 kb)
• 5 µg DNA binding capacity
• Gel extraction from standard or low-melt agarose gels in TAE or TBE buffer
• pH indicator dye in solubilization and binding buffer
• No loose resins or slurries to pipet
• No sodium iodide to interfere with subsequent reactions

pH indicator dye in the solubilization and binding buffer allows easy visual determination of optimal pH for DNA adsorption (pH ≤7.5). An incorrect binding-mixture pH can occur if the agarose gel electrophoresis buffer was frequently used or incorrectly prepared. In this case the pH can be easily adjusted by addition of 10 µl 3 M sodium acetate, pH 5.0.

操作手册: MinElute Handbook 04/01 (PDF version, 679 KB)