Further information:
MinElute and QIAquick DNA Cleanup Systems

QIAquick Gel Extraction Kit

For gel extraction or cleanup of DNA from enzymatic reactions

Application:	Gel extraction
Format:	Spin columns
Throughput:	1–24 samples
DNA size range:	70 bp – 10 kb*
Equipment required:	Microcentrifuge or vacuum manifold

* DNA fragments smaller than 70 bp or larger than 10 kb should be extracted with the QIAEX II Gel Extraction System.

Features and benefits

DNA fragments extracted in 15 minutes
70–80% recovery of fragments (70 bp – 10 kb) (see figure "High Recoveries from Gels")
Gel extraction from standard or low-melt agarose gels in TAE or TBE buffer
pH indicator dye in solubilization and binding buffer
No loose resins or slurries to pipet
No sodium iodide to interfere with subsequent reactions
Elution in as little as 30 µl Tris buffer or water

High Recoveries from Gels

DNA fragments (sizes indicated) before extraction with the QIAquick Gel Extraction Kit and pooled after extraction. Recoveries of approximately 80% are obtained for all fragment sizes. A 1–5: before extraction; P: pooled after extraction. Samples were analyzed on a 1.5% agarose gel in TAE buffer. B 1–3: before extraction; P: pooled after extraction. Samples were analyzed on a 3.5% highresolution–agarose gel in TAE buffer. M: pTZ-HinfI markers.

As with the MinElute Gel Extraction Kit, the solubilization and binding buffer in the QIAquick Gel Extraction Kit contains a unique pH indicator. A simple color change indicates whether the pH of the binding mixture is optimal for efficient adsorption of DNA to the QIAquick membrane (see figure "pH indicator dye"). The colored dye also allows easy visualization of any unsolubilized agarose in the binding mixture, ensuring complete solubilization for maximum yield.

pH indicator dye

pH indicator dye in the solubilization and binding buffer allows easy visual determination of optimal pH for DNA adsorption (pH ≤7.5). An incorrect binding-mixture pH can occur if the agarose gel electrophoresis buffer was frequently used or incorrectly prepared. In this case the pH can be easily adjusted by addition of 10 µl 3 M sodium acetate, pH 5.0.

Reliable Sequencing after Gel Extraction

Sequence of a 2.7 kb PCR product extracted from a TAE agarose gel with the QIAquick Gel Extraction Kit.

操作手册: QIAquick Spin Handbook 03/01 (PDF version, 643 KB)

Ordering Information
Product	Contents	Cat. No.
QIAquick Gel Extraction Kit (50)	50 QIAquick Spin Columns, Buffers, Collection Tubes (2 ml)	28704

QIAquick Gel Extraction Kit (250)	250 QIAquick Spin Columns, Buffers, Collection Tubes (2 ml)	28706

Buffer QG (250 ml)	250 ml Solubilization and Binding Buffer (with pH indicator)	19063

Buffer PE (concentrate, 100 ml)	100 ml Wash Buffer (5x concentrate for 500 ml buffer)	19065

† Buffer QG solubilizes agarose and can also be used with QIAquick 8 or 96 PCR Purification Kits for high-throughput gel extraction.