Further information:
QIAexpress Protein Purification System

Ni-NTA Agarose

For purification of 6xHis-tagged proteins by gravity-flow chromatography

Binding capacity:	5–10 mg/ml (300–500 nmol @ ~20 kDa)
Support:	Sepharose CL-6B
Bead structure:	Cross-linked, 6% agarose
Bead size:	45–165 µm
Form:	50% suspension in 30% ethanol, precharged with Ni2+

Ni-NTA Agarose provides Ni-NTA coupled to a Sepharose CL-6B support and offers high binding capacity and minimal nonspecific binding (see figure "One-Step Purification under Native Conditions"). This material has excellent handling properties for most scales of batch and column purification. Ni-NTA Agarose is available separately or as a component of QIAexpress Kits, which are complete kits for efficient expression and purification of 6xHis-tagged proteins from E. coli .

One-Step Purification under Native Conditions

Human serum response factor (SRF) was expressed in HeLa cells carrying a vaccinia virus vector and purified using Ni-NTA Agarose with different imidazole concentrations in the wash and elution steps. Proteins were visualized by silver staining. CL: cell lysate; FT: flow-through; W1: 0.8 mM wash; W2 & W3: 8 mM wash; W4 & W5: 40 mM wash; E1 & E2: 80 mM elution. (Data kindly provided by H. Stunnenberg, EMBL, Heidelberg, Germany.)

 操作手册: QIAexpress Detection and Assay Handbook 03/01 (PDF version, 450 KB)