Further information:
QIAexpress Protein Purification System

Ni-NTA Superflow

For purification of 6xHis-tagged proteins by FPLC

Binding capacity: 5¨C10 mg/ml (300¨C500 nmol @ ~20 kDa)
Support: Superflow
Bead structure: Highly cross-linked, 6% agarose
Bead size: 60¨C160 µm
Form: 50% suspension in 30% ethanol, precharged with Ni2+
   

Ni-NTA Superflow provides Ni-NTA coupled to Superflow resin and combines superior mechanical stability and outstanding flow characteristics with high dynamic binding capacity. This resin allows one-step purification of 6xHis-tagged proteins under high flow rates and pressures for efficient production-scale and FPLC applications (see figure "FPLC Purification with Ni-NTA Superflow").

FPLC Purification with Ni-NTA Superflow*


Elution with a step gradient using Ni-NTA Superflow. 6xHis-tagged chloramphenicol acetyltransferase was purified under native conditions on a 1 ml Ni-NTA Superflow column connected to an FPLC system at a flow rate of 1 ml/min. After applying the sample, the column was washed with 10 ml wash buffer (containing 20 mM imidazole) and eluted with 5 ml elution buffer (containing 200 mM imidazole). 1 ml fractions were collected and analyzed.  A  SDS-PAGE gel of eluate fractions. 11¨C15: eluate fractions; CL: cleared lysate; M: marker proteins.  B  Elution and gradient profile. Note: imidazole contributes to A280.

* Step gradients are recommended over linear gradients for better resolution and sharper elution peaks on Ni-NTA resins.

 ²Ù×÷ÊÖ²á: QIAexpress Detection and Assay Handbook 03/01 (PDF version, 450 KB)

Ordering Information
Product Contents Cat. No.
Ni-NTA Superflow (25 ml) 25 ml nickel-charged resin (max. pressure: 140 psi) 30410
Ni-NTA Superflow (100 ml) 100 ml nickel-charged resin (max. pressure: 140 psi) 30430
Ni-NTA Superflow (500 ml) 500 ml nickel-charged resin (max. pressure: 140 psi) 30450