NEW BioMag System

For magnetic cell separation and magnetocapture, immunoassays, and binding of immunoglobulins.

Features and benefits

Products

Principle

BioMag suspensions are a series of products for cell sorting and magnetocapture, enzyme and other immunoassays. BioMag particles are irregularly shaped, with an average diameter of 1.6 µm, and consist of an iron oxide core with a silane coating. The surface of the particle is coated with amine or carboxyl groups, facilitating the covalent attachment of proteins, glycoproteins, secondary antibodies, and other ligands, with retention of biological activity. The particles' irregular shape provides a large surface area ensuring a high binding efficiency.

BioMag particles are superparamagnetic, that is, they respond to magnetic fields but do not retain magnetic properties when removed from the magnetic field. This fully reversible magnetization permits magnetic extraction without magnetically induced aggregation. Rapid and efficient removal of BioMag particles from suspension is achieved by the application of an external magnetic field (see figure "BioMag particles on a Single Tube Magnet").

BioMag particles on a Single-Tube Magnet

Procedure

Magnetic separation is performed in an easy and simple batch procedure. After washing the appropriate amount of BioMag particles to remove the preservative, BioMag particles are mixed and incubated with the target molecules of interest. The magnetically labeled fraction is recovered by applying the vessel to an external magnet (>20 megaoersted). The magnetic fraction can be washed and isolated for subsequent use in downstream procedures, or the supernatant is recovered for further use.

Applications

BioMag secondary antibody suspensions provide a well-established and economical technology for indirect negative depletion in cell separation applications. BioMag streptavidin suspensions are available in standard or nuclease-free format, highly suited for applications in proteomics, cell biology, and nucleic acid purification. BioMag Protein A and Protein G suspensions are highly suited for immunoprecipitation applications.

BioMag Secondary Antibody Suspensions

BioMag secondary antibody suspensions are standard BioMag particles coated with polyclonal secondary antibodies. They allow the rapid and efficient purification of target cells from a heterogeneous cell population using the negative depletion method of cell sorting. BioMag secondary antibody suspensions are also highly suited for use in enzyme immunoassays and radioassays that utilize the appropriate monoclonal antibody.

Ordering Information
Product Contents Cat. No. 操作手册
BioMag Goat Anti-Mouse IgG (50 ml) BioMag goat anti-mouse IgG secondary antibody suspension (1 mg/ml) 310004 BioMag Goat Anti-Mouse IgG
BioMag Goat Anti-Mouse IgG (500 ml) BioMag goat anti-mouse IgG secondary antibody suspension (1 mg/ml) 310007 BioMag Goat Anti-Mouse IgG
BioMag Goat Anti-Mouse IgG Fc (50 ml) BioMag goat anti-mouse IgG (Fc-region specific) secondary antibody suspension (5 mg/ml) 310044 BioMag Goat Anti-Mouse IgG Fc
BioMag Goat Anti-Mouse IgG Fc (500 ml) BioMag goat anti-mouse IgG (Fc-region specific) secondary antibody suspension (5 mg/ml) 310047 BioMag Goat Anti-Mouse IgG Fc
BioMag Goat Anti-Mouse IgM (50 ml) BioMag goat anti-mouse IgM secondary antibody suspension (1 mg/ml) 310084 BioMag Goat Anti-Mouse IgM
BioMag Goat Anti-Mouse IgM (500 ml) BioMag goat anti-mouse IgM secondary antibody suspension (1 mg/ml) 310087 BioMag Goat Anti-Mouse IgM
BioMag Goat Anti-Rat IgG (50 ml) BioMag goat anti-rat IgG secondary antibody suspension (1 mg/ml) 310104 BioMag Goat Anti-Rat IgG
BioMag Goat Anti-Rat IgG (500 ml) BioMag goat anti-rat IgG secondary antibody suspension (1 mg/ml) 310107 BioMag Goat Anti-Rat IgG
BioMag Goat Anti-Rat IgG Fc (50 ml) BioMag goat anti-rat IgG (Fc-region specific) secondary antibody suspension (5 mg/ml) 310144 BioMag Goat Anti-Rat IgG Fc
BioMag Goat Anti-Rat IgG Fc (500 ml) BioMag goat anti-rat IgG (Fc-region specific) secondary antibody suspension (5 mg/ml) 310147 BioMag Goat Anti-Rat IgG Fc
BioMag Goat Anti-Rabbit IgG (50 ml) BioMag goat anti-rabbit IgG secondary antibody suspension (1 mg/ml) 310204 BioMag Goat Anti-Rabbit IgG
BioMag Goat Anti-Rabbit IgG (500 ml) BioMag goat anti-rabbit IgG secondary antibody suspension (1 mg/ml) 310207 BioMag Goat Anti-Rabbit IgG
BioMag Goat Anti-Human IgG (50 ml) BioMag goat anti-human IgG secondary antibody suspension (1 mg/ml) 310304 BioMag Goat Anti-Human IgG
BioMag Goat Anti-Human IgG Fc (50 ml) BioMag goat anti-human IgG (Fc-region specific) secondary antibody suspension (5 mg/ml) 310344 BioMag Goat Anti-Human IgG Fc
BioMag Sheep Anti-Fluorescein IgG (50 ml) BioMag sheep anti-fluorescein IgG secondary antibody suspension (1 mg/ml) 310704 BioMag Sheep Anti-Fluorescein IgG
BioMag Sheep Anti-Fluorescein IgG (500 ml) BioMag sheep anti-fluorescein IgG secondary antibody suspension (1 mg/ml) 310707 BioMag Sheep Anti-Fluorescein IgG

BioMag Streptavidin Suspensions

BioMag streptavidin suspensions are standard BioMag particles coated with streptavidin, and are highly suited for use in DNA probe assays, mRNA isolation, or for rapid separation of a biotinylated component in solution. BioMag streptavidin suspensions are also well suited for use in enzyme immunoassays and cell sorting. BioMag streptavidin suspensions are available in standard or nuclease-free formats.

Ordering Information
Product Contents Cat. No. 操作手册
BioMag Streptavidin (5 ml) BioMag streptavidin suspension (5 mg/ml) 311711 BioMag Streptavidin
BioMag Streptavidin (50 ml) BioMag streptavidin suspension (5 mg/ml) 311714 BioMag Streptavidin
BioMag Nuclease-Free Streptavidin (10 ml) BioMag nuclease-free streptavidin suspension (1 mg/ml) 311732 BioMag Nuclease-Free Streptavidin

BioMag Protein A and Protein G Suspensions

BioMag Protein A and Protein G suspensions are standard BioMag particles coated with Protein A or Protein G, and provide high affinity binding of immunoglobulins, or can be used for removal of Fc fragments during Fab fragment purification. BioMag Protein A and Protein G suspensions are also well suited for use in immunoassays.

Ordering Information
Product Contents Cat. No. 操作手册
BioMag Protein A (10 ml) BioMag Protein A suspension (5 mg/ml) 311802 BioMag Protein A
BioMag Protein G (10 ml) BioMag Protein G suspension (5 mg/ml) 311812 BioMag Protein G
Single-Tube Magnet Magnet for separating magnetic particles in a 1.5 ml or 2 ml tube 36910 Single Tube Magnet for separation of BioMag particles in a 1.5 ml or 2 ml tube
12-Tube Magnet Magnet for separating magnetic beads in 12 x 1.5 ml or 2 ml tubes 36912
15 ml/50 ml Tube Magnet Magnet for separating magnetic particles in 5 x 15 ml and 3 x 50 ml tubes 36935 15 ml/50 ml Tube Magnet for separation of BioMag particles in 15 ml or 50 ml tubes
Flask Magnet Magnet for separating magnetic particles in a cell culture flask 36937 Flask Magnet for separation of BioMag particles in cell culture flasks