For high-level expression of C-terminally 6xHis-tagged proteins with endogenous N-termini
| Position of 6xHis tag: | C-terminus |
| In-frame cloning required: | 5' and 3' |
| Vectors included: | pQE-60 and pQE-70 (insert with endogenous ATG is cloned in-frame using NcoI or SphI respectively) |
| Proteins recognized by: | Penta,His and Tetra,His Antibodies and HRP Conjugates |
荷恬返過: The QIAexpressionist 03/01 (PDF version, 499 KB)
|
||||||||||