lac O: lac operator
RBS: Ribosome-binding site
ATG: Start codon
6xHis: 6xHis tag sequence
MCS: Multiple cloning site
Stop Codons: In all 3 reading frames
Col E1: Col E1 origin of replication
Ampicillin: Ampicillin resistance gene
For high-level expression of N-terminally 6xHis-tagged proteins
| Vectors included: | pQE-9, pQE-30, pQE-31, pQE-32, and pQE-40 |
| Proteins recognized by: | Penta，His, RGS，His, and Tetra，His Antibodies and HRP Conjugates |
This vector set provides vectors for expression of N-terminally 6xHis-tagged proteins. pQE-30, pQE-31, and pQE-32 provide the multiple cloning site in all three reading frames while pQE-9 has an alternative, shorter multiple cloning site. pQE-40 is designed for expression of DHFR-fusion proteins and is recommended for expression of poorly expressed proteins or short peptides, which are often prone to proteolysis. DHFR enhances both stability and antigenicity. Since DHFR itself displays little immunogenicity in mouse and rat, DHFR-fusion proteins are ideal for epitope screening.
Click here for restriction maps of the multiple cloning sites in QIAexpress pQE vectors.
|
PT5:
T5 promoter lac O: lac operator RBS: Ribosome-binding site ATG: Start codon 6xHis: 6xHis tag sequence MCS: Multiple cloning site Stop Codons: In all 3 reading frames Col E1: Col E1 origin of replication Ampicillin: Ampicillin resistance gene |
荷恬返過: The QIAexpressionist 03/01 (PDF version, 499 KB)
|
||||||||||