For expression of proteins that allow His-tag removal using TAGZyme Enzymes
| Vectors included: | TAGZyme pQE-1 and pQE-2 |
| Proteins recognized by: |
See related products: TAGZyme Kit, TAGZyme DAPase Enzyme, TAGZyme Qcyclase/pGAPase Enzymes
Both TAGZyme pQE vectors encode an N-terminal 6xHis tag that possesses a sequence optimized for DAPase digestion and give excellent protein expression rates. For proteins that do not contain an intrinsic DAPase stop point, TAGZyme pQE-1 encodes for a glutamine residue between the His-tag sequence and the protein sequence, which serves as a DAPase stop point when converted to pyroglutamate by the action of excess Qcyclase Enzyme. For proteins that contain an intrinsic DAPase stop point, TAGZyme pQE-2 allows removal of the complete N-terminal tag irrespective of the cloning site of the DNA insert. In order to choose the right vector for your application, refer to the TAGZyme System on page 86, or contact QIAGEN Technical Services or your local distributor.
操作手册:TAGZyme Handbook 06/01 (PDF version, 203 KB)
Click here for restriction maps of the multiple cloning sites in QIAexpress pQE vectors.
* Proteins will only be recognized prior to His-tag removal.
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PT5: T5 promoter |
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