For high-level expression of proteins tagged with both 6xHis and Tag，100
| Vectors included: | pQE-100 DoubleTag |
| Proteins recognized by: |
Penta，His, RGS，His, Tetra，His Antibodies and HRP Conjugates, and Tag，100 Antibodies |
Proteins expressed using the pQE-100 DoubleTag Vector have a Tag，100 epitope at the C-terminus in addition to an N-terminal 6xHis tag. The Tag，100 epitope is a sequence from mammalian MAP kinase 2 consisting of 12 amino acids. The 6xHis tag allows immobilization of the proteins to Ni-NTA HisSorb Plates or Ni-NTA Magnetic Agarose Beads. The orientated immobilization of the protein means that the Tag，100, which is positioned at the opposite end of the protein, is easily accessible and readily detected by the Tag，100 Antibody, which recognizes the Tag，100 epitope. This is especially useful for screening procedures including ELISA, drug screening, and protein quantitation.
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PT5:
T5 promoter lac O: lac operator RBS: Ribosome-binding site ATG: Start codon 6xHis: 6xHis tag sequence MCS: Multiple cloning site Tag，100: Tag，100 epitope Stop Codons: In all 3 reading frames Col E1: Col E1 origin of replication Ampicillin: Ampicillin resistance gene |
荷恬返過:The QIAexpressionist 03/01 (PDF version, 499 KB)
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