Further information:
QIAexpress Expression System

pQE-TriSystem Vector

For parallel expression of 6xHis-tagged proteins in E. coli, mammalian cells, and baculovirus-infected insect cells using a single construct

The pQE-TriSystem vector contains CAG, T5, and p10 promoters that enable 6xHis-tagged protein expression in mammalian, E. coli, and baculovirus-infected insect cells, respectively. Preliminary studies can be carried out in bacterial expression systems, using the strong T5 promoter, which is recognized by E. coli polymerase, and allows efficient expression of proteins in any E. coli strain. If expression in mammalian or insect cells is required — for example to obtain post-translational modifications — the same construct can be used without the need for time-consuming subcloning procedures.

Click here for restriction maps of the multiple cloning sites in QIAexpress pQE vectors.

PT5: T5 promoter lac O: lac operator RBS: ribosome binding site ATG: start codon 8xHis: His tag sequence MCS: multiple cloning site Stop Codons: stop codons in all three reading frames Ampicillin: ampicillin resistance gene P CAG: CMV/actin/globin promoter P p10: p10 promoter Kozak: Kozak consensus sequence Termination Region: transcription terminator region lef2, 603/1629: flanking baculovirus sequences to permit generation of recombinant baculoviruses pUC ori: pUC origin of replication.

 操作手册: The QIAexpressionist (PDF version, 499 KB)