Further information:
QIAexpress
Detection Systems
For simple, direct detection of 6xHis-tagged proteins without secondary antibodies
| Detection: | 6xHis-tagged proteins with as few as two exposed histidine residues |
| Sensitivity in dot blots:* | 1–2 ng |
| Sensitivity in western blots:* | 2–5 ng |
| Working concentration: | 1/1000 dilution of stock solution |
| Cross-reactivity: | High concentrations of proteins with metal-binding motifs, e.g., the 31 kDa molecularweight standard bovine carbonic anhydrase, a metalloenzyme binding 1 molecule of zinc per molecule of protein |
| Form: | Lyophilized |
| Working solution: | 500 ml |
* Detection using chromogenic substrate. Sensitivity may vary with different proteins.
Ni-NTA Conjugates consist of Ni-NTA coupled to calf intestinal alkaline phosphatase (AP) or horseradish peroxidase (HRP). They can be used for direct, straightforward detection of any recombinant protein with an accessible 6xHis tag. They eliminate the need for secondary antibodies, thereby saving time in detection procedures. Ni-NTA Conjugates are recommended for use with chromogenic substrates, and for E. coli expression systems. The use of chemiluminescent substrates with Ni-NTA conjugates is not recommended.
操作手册: QIAexpress Detection and Assay Handbook 03/01 (PDF version, 450 KB)