Further information:
QIAexpress
Detection Systems
TagĦ¤100 Antibody
For sensitive detection of proteins
expressed from the pQE-100 DoubleTag Vector
| Detection: |
Proteins with the TagĦ¤100 |
| Sensitivity
in ELISA:* |
1¨C5 ng/ml |
| Isotype: |
Mouse IgG1 |
| Cross-reactivity: |
High concentrations of proteins with
metal-binding motifs, e.g., the 31 kDa molecularweight standard bovine
carbonic anhydrase, a metalloenzyme binding 1 molecule of zinc per
molecule of protein |
| Form: |
Lyophilized |
| Working
solution: |
1000-2500 ml |
The TagĦ¤100 Antibody detects proteins expressed from
the pQE-100 DoubleTag Vector. These proteins contain an N-terminal 6xHis tag and
a C-terminal TagĦ¤100 that allow oriented binding of the protein to Ni-NTA
HisSorb Plates or Ni-NTA Magnetic Beads via their 6xHis tags, and detection of
the well-exposed TagĦ¤100 using the TagĦ¤100 Antibody (see
figure "ELISA using Ni-NTA HisSorb Strips and TagĦ¤100 Antibody").
This system is ideal for ELISA procedures, for example for drug screening or
accurate protein quantitation.
ELISA using
Ni-NTA HisSorb Strips and TagĦ¤100 Antibody
 |
 |
 |
Thioredoxin tagged with 6xHis at the N-terminus and
with the TagĦ¤100 at the C-terminus was diluted in 0.2% BSA in PBS, pH
7.2, applied to Ni-NTA HisSorb Strips either with ( )
or without ( ) 25 µg/ml
NIH/3T3 lysate protein and incubated for 1.5 h at room temperature (RT).
The amount of immobilized protein was determined by incubating with the
TagĦ¤100 Antibody for 2 h at RT followed by peroxidase-conjugated
anti-mouse IgG secondary antibody for 1 h at RT and detected using
o-phenylenediamine as substrate. |
²Ù×÷ÊÖ²á: QIAexpress
Detection and Assay Handbook 03/01 (PDF version, 450 KB)
| Ordering
Information |
| Product |
Contents |
Cat. No. |
| TagĦ¤100
Antibody, BSA-free (100 µg) |
100 µg
mouse anti-TagĦ¤100 (lyophilized, BSA-free, for 1000¨C2500 ml
working solution for ELISA) |
34680 |
|
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