Further information:
QIAexpress
Detection Systems
For sensitive detection of 6xHis-tagged proteins
| Detection: | N-terminal, C-terminal, and internal 6xHis tags |
| Sensitivity in dot blots:* | 0.5 ng |
| Sensitivity in western blots:* | 2 ng |
| Isotype: | Mouse IgG1 |
| Cross-reactivity: | Negligible with crude E. coli, yeast, mammalian, or insect cell lysates |
| Form: | Lyophilized |
| Working solution: | 1000 ml (Anti,His Antibody Selector Kit: 30 ml of each antibody) |
* Detection using chromogenic substrate. Sensitivity may vary with different proteins.
All QIAexpress mouse monoclonal antibodies are produced under conditions that guarantee the highest purity and activity. Hybridoma cell culture is carried out under serum-free conditions, ensuring that the preparations are free of viruses, mycoplasma, and contaminating immunoglobulins. Purification by adsorption chromatography is performed entirely at physiological pH, yielding preparations with the highest proportion of native antibody.
The Anti,His Antibody Selector Kit contains 3 µg of each of the three Anti,His Antibodies, allowing economical comparison for new proteins and applications.
荷恬返過: QIAexpress Detection and Assay Handbook 03/01 (PDF version, 450 KB)