QIAamp 96 DNA Blood Kit
For high-throughput isolation of
genomic, mitochondrial, and viral DNA from blood and related body fluids
| Format and
processing: |
2 x 96-well plates processed by
centrifugation |
| Sample
sources: |
Fresh, frozen, and dried whole blood
(with common anticoagulants such as citrate, EDTA, and heparin);*
plasma; serum; buffy coat; bone marrow; lymphocytes; platelets; body
fluids |
| Sample size: |
Up to 200 µl |
| Preparation
time: |
192 samples in 2¨C3 hours |
| Typical
yield: |
6 µg per 200 µl healthy
whole blood |
| Elution
volume: |
50¨C200 µl |
The
QIAamp 96 DNA Blood Kit provides proven QIAamp technology in a convenient
96-well format for high-throughput purification needs. QIAamp 96 plates provide
well-to-well uniformity in DNA recovery and purity (see figures "Sample
Reproducibility" and "Reproducibility of
Yield and Purity"). As many as 192 samples can be processed in 2¨C3
hours using the centrifugation-based procedure, yielding highly pure DNA in less
than 1 minute per preparation. Even higher throughput can be achieved by
staggering the procedure.
The QIAamp 96 spin procedure requires the deep rotor
buckets of the QIAGEN
96-Well-Plate Centrifugation System to accommodate QIAamp 96 plates stacked
on 96-well blocks.
Sample
Reproducibility
Gel electrophoresis of 96 DNA
preparations purified from a single sample of whole human blood using the QIAamp
96 DNA Blood Kit. End lanes contain lambda¨C HindIII
markers.
Reproducibility
of Yield and Purity
 |
A
DNA yields (in µg) and B
purity of 96 preparations isolated from a single donor and purified
using the QIAamp 96 DNA Blood Kit. Mean values and standard errors from
ten such experiments are shown. |
²Ù×÷ÊÖ²á:
QIAamp 96 DNA Blood Kit Handbook 12/01
(PDF version, 416KB)
| Ordering
Information |
| Product |
Contents |
Cat.
No. |
| QIAamp
96 DNA Blood Kit (4) |
For 4 x 96
DNA preps: 4 QIAamp 96 Plates, QIAGEN Protease, Reagents,
Buffers, Lysis Blocks, Tape Pads, Collection Vessels |
51161 |
|
| QIAamp
96 DNA Blood Kit (12) |
For 12 x 96
DNA preps: 12 QIAamp 96 Plates, QIAGEN Protease, Reagents,
Buffers, Lysis Blocks, Tape Pads, Collection Vessels |
51162 |
|
