Further information:
QIAamp System

QIAamp DNA Blood Mini, Midi, and Maxi Kits

For isolation of genomic, mitochondrial, or viral DNA from blood and related body fluids

  QIAamp DNA Blood Mini Kit QIAamp DNA Blood Midi Kit QIAamp DNA Blood Maxi Kit
Format: Mini spin columns with 2.0 ml collection tubes Midi spin columns with 15 ml collection tubes Maxi spin columns with 50 ml collection tubes
Sample sources: Fresh and frozen whole blood (with common anticoagulants such as citrate, EDTA, and heparin); plasma; serum; buffy coat; bone marrow; lymphocytes; platelets; body fluids
Sample size: Up to 200 µl 1每2 ml 2每10 ml
Preparation time: 20每40 minutes 55 minutes 55 minutes
Typical yield (volume of healthy whole blood): 4每12 µg
(200 µl)		 20每60 µg
(1 ml)		 300每600 µg
(10 ml)
Elution volume: 50每200 µl 100每400 µl 500每2000 µl

QIAamp DNA Blood Mini, Midi, and Maxi Kits provide rapid DNA purification from 1 µl to 10 ml of blood. All QIAamp DNA Blood Kits use centrifugation-based purification procedures. The QIAamp DNA Blood Mini Kit can also be used with the QIAvac 24 or QIAvac 6S vacuum manifold. QIAamp DNA Blood Kits yield DNA sized from 200 bp to 50 kb depending on the age and storage of samples (see figure "Apoptotic Banding in Stored Blood"). The purified DNA is suitable for long-range PCR amplification (see figure "Long-Range PCR").

QIAamp DNA Blood Kits are supplied with a comprehensive handbook containing protocols for DNA isolation from a number of sample sources.

DNA Yields from Human Whole Blood with Different Cell Densities Using QIAamp DNA Blood Kits

  Midi (2 ml) Maxi (10 ml)
 
Leukocytes
per ml		 DNA yield (µg) DNA recovery (%) DNA yield (µg) DNA recovery (%)
2.5 x 105 3.0 92.3 15.8 95.8
1.0 x 106 11.0 91.7 60.4 91.5
5.0 x 106 62.4 94.5 312.0 94.5
1.0 x 107 116.3 88.1 624.6 94.6

Genomic DNA was purified from 2 ml (Midi) or 10 ml (Maxi) of human whole blood and eluted in 300 µl or 1 ml elution buffer, respectively. The first eluate was loaded onto the column a second time and centrifuged again (i.e., re-eluted). Percentage DNA recovery was calculated by assuming that one leukocyte contains 6 pg DNA.

Apoptotic Banding in Stored Blood

Genomic DNA from 8 blood samples stored at 4∼C for 1 week. DNA was purified using the QIAamp DNA Blood Mini Kit. When blood is stored at 4∼C the DNA is rapidly degraded due to apoptosis; the resulting apoptotic banding pattern can clearly be seen in these samples. M1: lambda每HindIII; M2:100 bp ladder.

Long-Range PCR

Amplification of a 10 kb fragment of the human ALDH1 gene from genomic DNA isolated from blood. DNA was purified using conventional methods (Phenol) or the QIAamp DNA Blood Maxi Kit (QIAamp). PCR products were sequenced directly. M: 1 kb ladder.

紱釬忒聊: QIAamp DNA Blood Midi Kit and QIAamp DNA Blood Maxi Kit Handbook 09/01(PDF version, 82KB)

Ordering Information
Product Contents Cat. No.
QIAamp DNA Blood Mini Kit (50) For 50 DNA minipreps: 50 QIAamp Mini Spin Columns, QIAGEN Protease, Reagents, Buffers, Collection Tubes (2 ml) 51104
QIAamp DNA Blood Mini Kit (250) For 250 DNA minipreps: 250 QIAamp Mini Spin Columns, QIAGEN Protease, Reagents, Buffers, Collection Tubes (2 ml) 51106
QIAamp DNA Blood Midi Kit (20)* For 20 DNA midipreps: 20 QIAamp Midi Spin Columns, QIAGEN Protease, Buffers, Collection Tubes (15 ml) 51183
QIAamp DNA Blood Midi Kit (100)* For 100 DNA midipreps: 100 QIAamp Midi Spin Columns, QIAGEN Protease, Buffers, Collection Tubes (15 ml) 51185
QIAamp DNA Blood Maxi Kit (10)* For 10 DNA maxipreps: 10 QIAamp Maxi Spin Columns, QIAGEN Protease, Buffers, Collection Tubes (50 ml) 51192
QIAamp DNA Blood Maxi Kit (50)* For 50 DNA maxipreps: 50 QIAamp Maxi Spin Columns, QIAGEN Protease, Buffers, Collection Tubes (50 ml) 51194

* Requires use of a centrifuge with swinging bucket rotors, able to centrifuge up to 4500 x g.