For isolation of genomic, mitochondrial, bacterial, parasite, or viral DNA
|
| Format: | Mini spin columns |
| Sample sources: | Muscle, liver, heart, brain, bone marrow, and other tissues; swabs (buccal, eye, nasal, pharyngeal, and others), CSF, blood, body fluids, washed cells from urine |
| Sample size: | Up to 50 mg tissue or up to 200 µl fluid |
| Hands-on
preparation time (after lysis): |
20 minutes |
| Typical yield: | See table "Yields with the QIAamp DNA Mini Kit" |
| Elution volume: | 50每200 µl |
The QIAamp DNA Mini Kit is ideal for purification of DNA from most commonly used human tissue samples, such as muscle, liver, heart, and brain. No mechanical homogenization is necessary as the tissues are lysed enzymatically, and the convenient spin-column procedure means that hands-on preparation time is only 20 minutes. Samples can be processed using either a microcentrifuge or, if blood or other body fluids are being processed, using the QIAvac 24 or QIAvac 6S vacuum manifold. In addition, the rigorous lysis procedure employed makes the QIAamp DNA Mini Kit ideal for purification of genomic DNA from bacteria or parasites. Table "Yields with the QIAamp DNA Mini Kit" shows typical DNA yields obtained from various tissue-sample types using the QIAamp DNA Mini Kit.
Yields with the QIAamp DNA Mini Kit
| Yield | |||
|
|
|||
| Sample | Total
nucleic acids (µg)* |
DNA (µg)† |
|
|
|
|||
| Blood | (200 µl) | 4每12 | 4每12 |
| Buffy coat | (200 µl) | 25每50 | 25每50 |
| Cells | (107) | 40每60 | 30每40 |
| Liver | (25 mg) | 60每115 | 10每30 |
| Brain | (25 mg) | 35每60 | 15每30 |
| Lung | (25 mg) | 25每45 | 5每10 |
| Heart | (25 mg) | 15每40 | 5每10 |
| Kidney | (25 mg) | 40每85 | 15每30 |
| Spleen | (10 mg) | 25每45 | 5每30 |
* Nucleic acids obtained without RNase
treatment
† Nucleic acids obtained with RNase
treatment
紱釬忒聊: QIAamp DNA Mini Kit and QIAamp DNA Blood Mini Kit Handbook 09/01 (PDF version, 520KB)