For isolation of genomic, bacterial, viral, and parasite DNA from stool
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| Format: | Mini spin columns |
| Sample sources: | Fresh or frozen stool |
| Sample size: | Up to 220 mg* |
| Preparation time: | 50 minutes |
| Typical yield: | 10¨C30 µg |
| Elution volume: | 200 µl |
* Protocol for larger amounts of stool requires additional Buffer ASL.
The QIAamp DNA Stool Mini Kit allows rapid purification of DNA from fresh or frozen human stool or other sample types with high concentrations of PCR inhibitors (see figure "Complete Removal of Inhibitors Enables PCR Amplification"). Highly pure DNA ready for direct use in downstream amplification reactions is purified in about 50 minutes using the convenient QIAamp spin-column procedure. PCR inhibitors are removed by the combined action of InhibitEX, a unique adsorption resin, and an optimized buffer. Rigorous lysis using Proteinase K ensures high yields of all types of DNA common in stool, including colorectal epithelial cells, bacteria, viruses, and other gastrointestinal pathogens. Remaining impurities are efficiently removed in two wash steps. Amplification-ready DNA is then eluted in low-salt buffer.
Complete Removal of Inhibitors Enables PCR Amplification
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DNA was purified from 19 stool samples using A a conventional silica-based purification technique and B the QIAamp DNA Stool Mini Kit. To show whether inhibitors were present in the purified eluates, 5 µl of each eluate was added to PCR mixes with a template of 10 pg plasmid GFP) gene. Amplification of the GFP gene was succescontaining the green fluorescent protein (sful in the presence of all QIAamp eluates whereas only 2 amplification reactions were successful in the presence of eluates prepared using the conventional technique. M: markers; C: positive PCR control. |
²Ù×÷ÊÖ²á:QIAamp DNA Stool Mini Kit Handbook 08/01 (PDF version, 168KB)
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* Protocol for larger amounts of stool requires additional Buffer ASL. |