Further information:
QIAamp System

QIAamp UltraSens Virus Kit

For concentration and isolation of viral RNA and DNA from serum and plasma

Format: Mini spin columns
Sample sources: Plasma, serum
Sample size: 1 ml
Preparation time: 1 hour
Typical yield: >85% recovery of nucleic acids
Elution volume: 60 µl

The QIAamp UltraSens Virus Kit uses new technology to concentrate viral nucleic acids in plasma and serum samples, followed by nucleic acid purification using proven QIAamp technology. The procedure provides increased sensitivity in viral-load monitoring and other applications where high viral nucleic acid recovery is essential. Nucleic acid concentration is achieved by first adding a novel reagent to the sample. The reagent forms complexes with nucleic acids, allowing them to be highly concentrated by low-speed centrifugation. This step allows nucleic acid purification from large sample volumes without the inconvenience of handling large volumes throughout the protocol. Viral nucleic acids are then purified using QIAamp silica-gel-membrane technology. No ultracentrifugation or specialized laboratory equipment is required, and an internal control can be added at the start of the procedure, allowing full monitoring of the purification process. The kit is suitable for purification of viral nucleic acids from a wide range of viral species (see table "UltraSens Technology Facilitates Highly Sensitive Detection of Viruses"). It can also be used for efficient isolation of extracellular genomic DNA from plasma and serum. The purified nucleic acids perform well in sensitive downstream applications (see figure "High Performance of Purified Nucleic Acids in Downstream Analyses").

High Performance of Purified Nucleic Acids in Downstream Analyses

RNA purified from HAV (NIBSC HAV RNA Working Reagent) using the QIAamp UltraSens procedure was used in  A  one-step RT-PCR and in  B  quantitative, real-time RT-PCR with the LightCycler. geq/ml: genome equivalents per milliliter; ¨C: negative control; M: markers. Data kindly provided by Dr. Thomas Laue, Artus GmbH, Hamburg, Germany.

UltraSens Technology Facilitates Highly Sensitive Detection of Viruses

Virus
Detection limit
Detection method*
HCV
1.8 IU/ml
AMPLICOR
HIV
10 IU/ml
AMPLICOR
HBV
15 genome equivalents/ml
TaqMan
HAV
4 genome equivalents/ml†
TaqMan
B19V
16 genome equivalents/ml
TaqMan

* Viral nucleic acids were amplified and detected using either the Cobas AMPLICORor TaqMan systems from Roche Molecular Systems.
† Detection limit depends on the standard used.

²Ù×÷ÊÖ²á: QIAamp UltraSens Virus Kit Handbook 12/00 (PDF version, 178KB)

Ordering Information
Product Contents Cat. No.
QIAamp UltraSens Virus Kit (50) For 50 viral nucleic acid preps: 50 QIAamp Mini Spin Columns, Proteinase K, Carrier RNA, Collection Tubes (2 ml), Buffers 53704
QIAamp UltraSens Virus Kit (250) For 250 viral nucleic acid preps: 250 QIAamp Mini Spin Columns, Proteinase K, Carrier RNA, Collection Tubes (2 ml), Buffers 53706