Further information:
DNeasy Plant System

DNeasy Plant Mini and Maxi Kits

For isolation of total cellular DNA from plant cells and tissues, or fungi

	DNeasy Plant Mini Kit		DNeasy Plant Maxi Kit
Format:	Mini spin columns with 2 ml collection tubes		Maxi spin columns with 50 ml collection tubes
Sample source:	Plant cells and tissues, fungi		Plant cells and tissues, fungi
Sample size:	Up to 100 mg wet weight		Up to 1 g wet weight
Preparation time:	<1 hour		<2 hours
Typical yield:*	3–30 µg		30–260 µg
Elution volume:	50–400 µl		500 µl – 2 ml

* DNA yields vary between different species and tissues depending on genome size, ploidy, cell number, and age of tissue sample. Lower and higher range values correspond to arabidopsis and wheat, respectively.

DNeasy Plant Mini and Maxi Kits allow rapid and efficient isolation of high-quality DNA from a wide variety of plant species and tissue types including the most demanding sources (see table "Selection of plant species processed with DNeasy Kits"). Samples may be fresh, frozen, or dried. The optimized DNeasy Plant procedure incorporates QIAshredder, a unique filtration and homogenization column that efficiently removes cell debris and improves sample handling following lysis.

The DNeasy Plant procedure yields pure nucleic acid, free of polysaccharides and other secondary metabolites often copurified using conventional methods. Such impurities can interfere with spectrophotometric readings and inhibit enzymatic reactions. DNeasy purified DNA is sized up to 40kb (see figure "Pure DNA (20–25 kb) forRestriction Analysis"), and is suitable for downstream applications such as PCR, Southern blotting, RAPD, AFLP, and RFLP analysis (see figures "RAPD Analysis of Sunflower Species", "Comparison of CTAB and DNeasy Methods: PCR Performance", and "PCR Analysis of DNA from Different Plant Species").

Selection of plant species processed with DNeasy Kits

Young leaves or needles (and other tissues, as indicated) were collected and immediately flash frozen. DNA isolation was then performed with the DNeasy Plant Mini Kit. 1Beechnut, 2dried leaves, 3callus, 4leaves from adult plant, 5endosperm, 6old leaves, rich in carbohydrates, 7buds.

Pure DNA (20–25 kb) forRestriction Analysis

Agarose gel (0.8%) analysis of DNA isolated from the indicated leaves or needles with the DNeasy Plant Mini Kit. 0.5 µg of undigested (left) or 1 µg of EcoRI-digested DNA (right) were loaded in each pair of lanes. M: lambda– HindIII.

RAPD Analysis of Sunflower Species

DNA (50 ng) from leaves of the indicated in vitro-propagated sunflower (Helianthus) species was amplified using a 10-base RAPD primer and separated on a 1.5% agarose gel. M: 100 bp ladder. (Data kindly provided by H. J. Henn, Institute of Agricultural Botany, University of Bonn, Germany.)

Comparison of CTAB and DNeasy Methods: PCR Performance

DNA was isolated from arabidopsis leaves using either CTAB lysis (CTAB) or the DNeasy Plant Maxi Kit (DNeasy). Amplification reactions were prepared using purified DNA (1: 50 pg; 2: 100 pg) and primers to the Akin 10 gene. M: markers. (Data kindly provided by Alain Lecharny, Institut de Biotechnologie des Plantes, UMR CNRS-UPS Orsay, France.)

PCR Analysis of DNA from Different Plant Species

DNA (10 ng) from the indicated leaves or needles was amplified using universal primers for the noncoding intergenic spacer between the tRNA genes trnL (UAA) 5' exon and trnL (UAA) 3' exon of cpDNA (Taberlet, P. et al. (1991) Plant Mol. Biol. 17, 1105). M: 100 bp ladder.

操作手册:
DNeasy Plant Mini Kit and DNeasy Plant Maxi Kit Handbook 08/00 (complete PDF version, 135 KB)