Further information:
DNeasy Plant System

DNeasy 96 Plant Kit

For high-throughput isolation of total cellular DNA from plant leaf tissue

*DNA yields vary between species depending on genome size, ploidy, cell number, and age of tissue sample.

The DNeasy 96 Plant Kit provides high-throughput isolation of high-quality DNA from 96 or 192 samples of plant leaf tissue in less than 2 hours. The simple 96-well procedure provides highly reproducible yields of total cellular DNA (see figures "Uniformity of DNA Yields from 96 Wheat Samples" and "Reproducible DNA Yield and Quality"). The streamlined DNeasy procedure and convenient 96-well format (see flowchart "DNeasy Plant Procedure and DNeasy 96 Plant Procedure") eliminate tedious labeling, one-at-a-time pipetting, and DNA resuspension steps, and help minimize cross-contamination (see figure "Reproducible DNA Yield and Quality"). Polysaccharides, proteins, and enzyme inhibitors are efficiently removed, making DNeasy purified DNA suitable for use in all downstream applications, such as PCR (see figure "Reliable PCR from Different Plant Species"), AFLP (see figure "AFLP Analysis of Eucalyptus DNA"), RAPD, and Southern blotting. Processing of DNeasy 96-well plates requires use of the QIAGEN 96-Well-Plate Centrifugation System.

AFLP Analysis of Eucalyptus DNA

Genomic DNA was purified from 2 eucalyptus trees (first 2 lanes) and their progeny (remaining lanes). After digestion with EcoRI and MseI, adapters were ligated to the ends of the fragments and adapter-specific primers were used to amplify AFLP fragments in 2 rounds of PCR. (Data kindly provided by A. Myburg, Forest Biotechnology Group, NC State University, Raleigh, NC.)

Uniformity of DNA Yields from 96 Wheat Samples

96 leaf samples were collected from a batch of wheat plants grown under identical conditions. 50 mg samples were disrupted in liquid nitrogen and DNA was isolated using the DNeasy 96 Plant procedure.

High-throughput sample disruption

For rapid, efficient disruption of up to 192 plant tissue samples in just 2–4 minutes, QIAGEN offers the first commercially available high-throughput disruption system — the Mixer Mill MM 300. The Mixer Mill MM 300 uniformly disrupts samples allowing reproducible, high-quality results in downstream applications such as genomic DNA isolation from plant tissues.

Reproducible DNA Yield and Quality

Genomic DNA was purified from different plant species following disruption in liquid nitrogen. DNA was eluted in 2 x 100 µl Buffer AE. 10 µl of each eluate was loaded per lane. Average yields are given under the plant names. M: lambda–HindIII.

Reliable PCR from Different Plant Species

Genomic DNA purified from different plant species was amplified by PCR. 5 µl of each eluate was used as template, and 30 µl of each PCR product was loaded per lane. M: markers.

DNeasy 96 Plant Kit Handbook 02/02 (complete PDF version, 569 KB)