Further information:
DNeasy Tissue System
For isolation of genomic DNA from animal tissues and cells, yeast, or bacteria
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Format:
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Mini spin columns with 2 ml collection tubes
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Sample source:
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Animal tissues and cells, yeast, or bacteria
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Sample size:
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Up to 25 mg tissue or 1.2 cm rodent tail
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Throughput:
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1每24 samples
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Preparation time:
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20 minutes after lysis
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Typical yield:*
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20每40 µg
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Elution volume:
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50每400 µl
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* From animal tissues and cells
The DNeasy Tissue Kit provides rapid isolation of genomic DNA from a wide range of organisms (see figure "DNA Isolation from Distantly Related Organisms") and fresh and frozen animal tissues (see figure "DNA Isolation from Various Mammalian Tissues"). For convenience and reduced hands-on time, cells are directly lysed with QIAGEN Proteinase K without the need for mechanical homogenization. After lysis, DNA is purified in as little as 20 minutes using the simple DNeasy procedure in a convenient spin-column format. Special protocols and guidelines are provided for different samples, such as paraffinembedded tissue, blood, cultured cells, rodent tails, insects, bacteria, and yeast.
DNA Isolation from Distantly Related Organisms
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Agarose gel analysis of genomic DNA from the indicated species prepared with the DNeasy Tissue Kit. 0.5 µg DNA was analyzed undigested (left) and 1 µg after digestion with EcoRI (right). M: lambda每HindIII. |
DNA Isolation from Various Mammalian Tissues
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Agarose gel analysis of genomic DNA from the indicated tissues of the espective mammals prepared with the DNeasy Tissue Kit. For each sample, 0.5 µg DNA was analyzed undigested (left) and 1 µg after digestion with EcoRI (right). M: lambda每HindIII. |
紱釬忒聊: DNeasy Tissue Kit Handbook 05/02 (complete PDF version, 199 KB)