Further information:
DNeasy Tissue System
For high-throughput isolation of genomic DNA from animal tissues and cells
| Format: | 96-well plates |
| Sample source: | Animal tissues and cells* |
| Sample size: | Up to 1.2 cm rodent tail or 20 mg tissue |
| Throughput: | Up to 2 x 96 samples |
| Preparation time: | 1 hour after lysis |
| Typical yield: | 20¨C30 µg |
| Elution volume: | 50¨C400 µl |
The DNeasy 96 Tissue Kit is designed for rapid, high-throughput DNA isolation from rodent tails (see figure "High-Quality DNA from Every Sample") and other tissues. Direct lysis of tissue samples with QIAGEN Proteinase K avoids mechanical homogenization and reduces hands-on time (see below). The efficient DNeasy procedure and convenient 96-well format (see DNeasy Tissue System) eliminate the need for organic extraction, labeling of individual tubes, one-at-a-time pipetting, and DNA resuspension so that downstream applications such as PCR (see figure "Reliable PCR from Every Sample") can be completed on the same day.
Processing of DNeasy 96-well plates requires the use of the QIAGEN 96-Well-Plate Centrifugation System.
High-Quality DNA from Every Sample
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Genomic DNA isolated from 36 mouse tails with the DNeasy 96 Tissue Kit. From each 400 µl eluate, 30 µl was loaded on a 1% agarose gel. Markers: lambda¨CHindIII. |
Reliable PCR from Every Sample
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PCR amplification of a 500 bp sequence from the p53 gene in DNA samples isolated from 48 mouse tails. From each 100 µl reaction, 10 µl was loaded on a 1% agarose gel. Markers: Low DNA Mass Ladder. |
²Ù×÷ÊÖ²á: DNeasy 96 Tissue Kit Handbook 02/02 (complete PDF version, 596 KB)
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* Requires use of the QIAGEN
96-Well-Plate Centrifugation System |