Further information:
Oligotex mRNA Purification System

Oligotex mRNA Kits

For isolation of poly A+ mRNA from total RNA and cleanup of in vitro transcripts

Amount of starting
material per prep
Maximum number of preps per kit
(spin-column protocol)*
Total RNA
Mini
Midi
Maxi
< 0.25 mg 12 (13)† 12 (28)† 6 (17)†
0.25每0.50 mg 6 12 (14)† 6 (14)†
0.50每0.75 mg 4 12 (14)† 6 (14)†
0.75每1.00 mg 3 12 6 (12)†
1.0每1.5 mg 每 每 6 (8)†
1.5每2.0 mg 每 每 6
2.0每2.5 mg 每 每 5
2.5每3.0 mg 每 每 4

* Batch protocols also available
† Maximum number of preps per kit with purchase of additional spin columns. Additional 1.5 ml microcentrifuge tubes may be required.

Oligotex mRNA Kits contain spin columns and all necessary reagents and buffers for isolation of pure poly A+ mRNA from total RNA preparations or cleanup of in vitro transcripts. Poly A+ mRNA purification takes less than 30 minutes with >90% recovery. Poly A+ mRNA is immediately ready for all standard applications, as well as for sensitive applications such as expression array, differential display, cDNA library construction, microinjection, and SAGE™ technology. Oligotex Suspension is also available separately.

紱釬忒聊: Oligotex Handbook 05/02 (PDF version, 357 KB)

Ordering Information
Product Contents Cat. No.
Oligotex mRNA Mini Kit (12) For 12 mRNA minipreps: 200 µl Oligotex Suspension, Small Spin Columns, Collection Tubes (1.5 ml), RNase-free Reagents and Buffers 70022
Oligotex mRNA Midi Kit (12) For 12 mRNA midipreps: 700 µl Oligotex Suspension, Small Spin Columns, Collection Tubes (1.5 ml), RNase-free Reagents and Buffers 70042
Oligotex mRNA Maxi Kit (6) For 6 mRNA maxipreps: 700 µl Oligotex Suspension, Large Spin Columns, Collection Tubes (1.5 ml), RNase-free Reagents and Buffers 70061
Oligotex Suspension (0.5 ml) 0.5 ml for mRNA isolation from up to 8 mg of total RNA 79000
Oligotex Suspension (2.5 ml) 2.5 ml for mRNA isolation from up to 40 mg of total RNA 79002
Small Spin Columns (24) 24 RNase-free spin columns for Oligotex spin procedures 79523