Further information:
RNeasy Stabilization and Total RNA Isolation System

RNeasy Protect Mini, Midi, and Maxi Kits

For RNAlater stabilization and RNeasy purification of RNA from animal tissues

	RNeasy Protect Mini Kit	RNeasy Protect Midi Kit	RNeasy Protect Maxi Kit
Format:	Mini columns with 1.5 ml and 2 ml collection tubes	Midi columns with 15 ml collection tubes	Maxi columns with 50 ml collection tubes
Sample size
Tissue:	0.5–30 mg	20–250 mg	150 mg – 1 g
Volume of RNAlater Stabilization Reagent:†	50 ml (50-prep kit) 250 ml (250-prep kit)	20 ml (10-prep kit) or 100 ml (50-prep kit)	100 ml (12-prep kit)
Binding capacity:	Up to 100 µg RNA	Up to 1 mg RNA	Up to 6 mg RNA
Yield:	Varies depending on sample source. See table
Elution volume:	30–100 µl	300–500 µl	800–2400 µl

The RNeasy Protect system provides a complete RNA protection and isolation solution, from sample harvesting to pure RNA, in one kit. Proven RNeasy silica-gel–membrane technology, combined with the RNA-stabilizing properties of RNAlater RNA Stabilization Reagent, allows purification of high-quality, intact RNA (see figure "RNAlater Reagent Prevents Degradation of mRNA in Tissues"). This process efficiently preserves the expression profile of the sample at the time of harvesting to ensure reliable gene-expression analysis.

The innovative, aqueous RNAlater RNA Stabilization Reagent quickly permeates tissues† and other biological materials,* stabilizing and protecting the RNA expression pattern. Immediate protection with RNAlater technology ensures that downstream analyses truly reflect the expression profile of the intact tissues. Samples can be archived without risk of RNA degradation, even after multiple freeze–thaw cycles. RNeasy purified RNA from RNAlater stabilized samples is ideal for downstream processes, such as northern blotting and RT-PCR, from samples stored under a wide variety of conditions (see figure "RNA Stability in Tissues: Different Temperatures and Freeze–Thaw Cycles").

RNeasy Kits provide the highest-quality RNA with minimum copurification of DNA. For certain RNA applications that are sensitive to very small amounts of DNA, the residual amounts of DNA remaining can be removed using the RNase-Free DNase Set for convenient on-column DNase treatment during the RNeasy procedure.

* Can be used with other biological materials, such as eukaryotic cell-culture cells or white blood cells
† Stabilization requires 10 µl per 1 mg tissue.

RNAlater Reagent Prevents Degradation of mRNA in Tissues

RNA was isolated from fresh rat kidney samples after 0, 5, 10, 15, 30, and 60 minutes, using either standard procedures (left panel) or RNeasy Protect Kits (right panel). The RNA isolated was analyzed by agarose gel electrophoresis and expression of GAPDH was examined using northern blot analysis. M: markers.

RNA Stability in Tissues: Different Temperatures and Freeze–Thaw Cycles

Total RNA was isolated from tissue samples stored in RNAlater RNA Stabilization Reagent.  A  Rat lung tissue was stored under the indicated conditions. RNA was analyzed on a formaldehyde agarose gel and northern blot (GAPDH-probed).  B  Stabilized mouse liver tissue was subjected to freeze–thaw cycles (–20°C/25°C). C: Control tissues frozen in liquid nitrogen and stored at –80°C.

Total RNA yields obtained with RNeasy Protect Mini, Midi, and Maxi Kits

Amounts can vary due to developmental stage, species, growth conditions used, etc. Since the RNeasy procedure enriches for RNA species >200 nt, RNA yield does not include 5S rRNA, tRNAs, or other low-molecular-weight RNAs.

‡ Using the specialized protocol for heart, muscle, and skin tissue, included in handbook. Proteinase K (required for this protocol) is not provided.

操作手册:RNAlater Handbook 07/02 (PDF version, 118 KB)

         RNeasy Mini Handbook 6/01 (PDF version, 351 KB)

         RNeasy Midi/Maxi Handbook 06/01 (PDF version, 345 KB)