Further information:
RNAlater and RNAprotect Systems

NEW RNAlater TissueProtect Tubes and RNAlater RNA Stabilization Reagent

For stabilization of gene expression profiles in animal tissues

The innovative, aqueous RNAlater RNA Stabilization Reagent quickly permeates tissues, stabilizing and protecting the RNA expression pattern (see figure "RNAlater Reagent Prevents Degradation of mRNA in Tissues"). Immediate protection with RNAlater technology ensures that downstream analyses truly reflect the expression profile of the intact tissues (see figure "RNAlater TissueProtect Tubes Prevent RNA Changes in Tissues"). Samples can be archived without risk of RNA degradation, even after multiple freeze–thaw cycles. RNeasy purified RNA from RNAlater stabilized samples is ideal for downstream processes, such as northern blotting and RT-PCR, from samples stored under a wide variety of conditions (see figure "Stable RNA in Tissues: Different Temperatures and Freeze–Thaw Cycles").

RNAlater Reagent Prevents Degradation of mRNA in Tissues

RNA was isolated from fresh rat kidney samples after 0, 5, 10, 15, 30, and 60 minutes, using either standard procedures (Unstabilized) or RNeasy Protect Kits (Stabilized). The RNA isolated was analyzed by agarose gel electrophoresis and expression of GAPDH was examined using northern blot analysis. M: markers.

RNAlater TissueProtect Tubes Prevent RNA Changes in Tissues

Rat tissues (10 mg each) were excised and stored at room temperature for up to 4 hours in phosphate-buffered saline (PBS) or RNAlater RNA Stabilization Reagent (RNAlater). At the indicated times, RNA was isolated using the RNeasy Protect Mini Kit. Total RNA was used for quantitative RT-PCR using the QuantiTect Probe RT-PCR Kit and primers and probe specific for the transforming growth factor β (TGF-β) gene or the tumor necrosis factor α (TNF-α) gene. Analyses were carried out in triplicate on the ABI PRISM 7700 Sequence Detection System. The change in threshold cycle (CT) is shown, relative to the CT at time zero.

Stable RNA in Tissues: Different Temperatures and Freeze–Thaw Cycles

Total RNA was isolated from tissue samples stored in RNAlater RNA Stabilization Reagent.  A  Rat lung tissue was stored under the indicated conditions. RNA was analyzed on a formaldehyde agarose gel and northern blot (GAPDH-probed).  A  Stabilized mouse liver tissue was subjected to freeze-thaw cycles (-20°C/25°C). C: Control tissues frozen in liquid nitrogen and stored at -80°C.

操作手册: RNAlater Handbook 07/02 (PDF version, 118 KB)