RNase-Free DNase Set

The RNase-Free DNase Set provides reagents for DNase digestion during RNA purification.

Activity:	Lyophilized enzyme with RNase-Free Buffer and water
Format:	2500 Kunitz units/mg
Unit definition:	One Kunitz unit is defined as the amount of DNase I that causes an increase in A260 of 0.001 per minute per milliliter at 25°C, pH 5.0, with highly polymerized DNA as the substrate .
Reaction time:	15 minutes on-column at 20–30°C

Features and benefits

Guaranteed RNase-free
Stable, lyophilized enzyme
Convenient, on-column treatment and subsequent DNase removal
Optimized for use in QIAGEN spin-column RNA purification procedures

Applications

The QIAGEN RNase-Free DNase Set is quality-controlled and optimized for use with RNeasy procedures and the QIAamp RNA Blood Mini Kitprocedures. Generally, DNase digestion is not required for RNA purified with RNeasy Kits since the silica-gel–membrane, spin-column technology efficiently removes the majority of the DNA without DNase treatment. However, more complete DNA removal may be necessary for certain RNA applications that are sensitive to very small amounts of DNA.
The RNase-Free DNase Set provides efficient on-column digestion* of DNA during RNA purification from cells and tissues using RNeasy Kits and the QIAamp RNA Blood Mini Kit. The DNase is efficiently removed in subsequent wash steps.
For DNase treatment when using the RNeasy 96 Kit, please contact QIAGEN Technical Services or your local distributor for a separate, optimized protocol.

* The DNA digest buffer is not optimized for digestion in solution.

操作手册: RNase-Free DNase Set (PDF version, 71 KB)

Ordering Information
Product	Contents	Cat. No.
RNase-Free DNase Set (50)	1500 units RNase-free DNase I, RNase-free Buffer RDD, and RNase-free water for 50 RNA minipreps	79254