Further information:
QIAexpress
Detection Systems
For sensitive detection of biotinylated proteins
| Detection: | Biotinylated biomolecules* |
| Form: | Stabilized stock solution |
| Working solution: | Dependent on application |
For use in conjunction with the Penta·His Biotin Conjugate, QIAGEN offers streptavidin conjugated to the intensely fluorescing algal pigment R-phycoerythrin (Streptavidin-R-PE). By utilizing the extremely high affinity of the streptavidin-biotin interaction and the spectroscopic properties of R-phycoerythrin, the sensitivity of immunoassays can be increased some five- to tenfold over the corresponding Alexa 532 Conjugate. The high quantum yield (0.82) and extinction coefficient (2 x 106) of R-PE make it one of the best fluorophores currently available for high-sensitivity detection. Streptavidin-R-PE is highly suited for detection of biotinylated biomolecules using the LabMAP bead-based assay system.
* Streptavidin conjugates may also detect the endogenous, biotinylated E. coli biotin carboxyl carrier protein (BCCP) in crude cell lysates.
操作手册: QIAexpress Detection and Assay Handbook 03/01 (PDF version, 450 KB)
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