For automated purification of genomic and mitochondrial DNA from blood and other body fluids
| Format : | 96-well plates (for up to 96 preps) | |
| Sample sources: | Fresh or frozen whole blood (with common anticoagulants such as citrate and EDTA), serum, bone marrow, other body fluids, and cell suspensions | |
| Sample size: | 200 µl | |
| Preparation time: | <2 hours | |
| Typical yield: | 6 µg per 200 µl healthy whole blood | |
| Elution volume: | 200 µl | |
The
QIAamp 96 DNA Blood BioRobot Kit provides automated high-throughput DNA
purification on the BioRobot 9604 using proven QIAamp technology. DNA from up to
96 samples of human whole blood or other body fluids is purified in under 2
hours with minimal hands-on time. QIAamp 96-well plates provide uniform DNA
recovery and purity (see figure "Reproducible
DNA Yield and Purity"), and PCR experiments show no cross-contamination
between samples in adjacent wells (see figure "Cross-Contamination¨CFree
Purification for Reliable PCR"). QIAamp purified DNA is free of
contaminants and enzyme inhibitors, and is ready for use in almost any
amplification, Southern blotting, or other enzymatic assay (see figures "HLA
Typing" and "Factor V Analysis") in
applications such as HLA typing, genetic disease testing, identity testing, and
cancer screening. The automated QIAamp 96 DNA Blood procedure on the BioRobot
9604 helps to increase productivity and reliability in these applications.
Reproducible DNA Yield and Purity
  |
A Yield and B purity (measured in water) of 20 typical DNA preps from 96 preps purified from a single sample of human whole blood using the QIAamp 96 DNA Blood BioRobot procedure. Mean values and standard errors represent all 96 preps from the QIAamp 96 plate.
Cross-Contamination¨CFree Purification for Reliable PCR
 |
Cross-contamination control analysis of the automated DNA purification procedure on the BioRobot 9604. 200 µl samples of blood or water were added alternately to wells in the QIAamp 96 plate and the entire 96-well plate was processed following the QIAamp 96 DNA Blood BioRobot procedure. 5 µl of each 200 µl eluate was submitted to amplification in a 50 µl PCR using HotStarTaq DNA Polymerase, and the resultant products were analyzed on an agarose gel. No products were detected from eluates corresponding to wells containing water samples. Markers: Low DNA Mass Ladder (Gibco BRL). |
 |
SSP-PCR analysis of donor DNA using primers specific for HLA genes DRB (lanes 1¨C23) and DQB (lanes 24¨C45). Genomic DNA was isolated from human whole blood using the QIAamp 96 DNA Blood BioRobot procedure and amplified with 45 different allele-specific primers targeted at genes DRB1 through to DRB5, and DQB1. Allele-specific amplicons appear as intense bands below the 439 bp control amplicon (human growth hormone gene) in lanes 8, 9, 22, 37, 38, and 40. (Data kindly provided by Dr. S. Ferencik, Institut f¨¹r Immunologie [Director Prof. Dr. med. H. Grosse-Wilde] Universitätsklinikum Essen, Germany.) |
 |
Factor V Leiden genotyping using LightCycler technology. The figure shows two peaks corresponding to the DNA melting temperatures recorded for the wild-type allele (64¡ãC) and the mutant allele (57¡ãC) in a heterozygotic individual (red line) and a control DNA (blue line). Genomic DNA was isolated from human whole blood using the QIAamp 96 DNA Blood BioRobot procedure. (Data kindly provided by C. Lars Mouritsen and D. Hall, ARUP, Salt Lake City, UT, USA.) |
²Ù×÷ÊÖ²á: QIAamp DNA Blood BioRobot 9604 Kit Handbook 11/01 (PDF version, 444KB)