LiquiChip detection reagents are designed for simple and sensitive detection and quantification of bead-bound assay components. Penta，His Alexa Fluor Conjugates, Penta，His Biotin Conjugates, and Streptavidin-R-PE enable highly flexible and sensitive detection of analytes in LiquiChip assays.
LiquiChip Applications Handbook 04/02 (PDF version, 325 KB)
The following detection reagents are used with the LiquiChip system:
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Penta，His Alexa Fluor 532 Conjugate consists of mouse monoclonal Penta，His Antibodies conjugated to the fluorophore Alexa Fluor 532, and can be used for direct detection of 6xHis-tagged proteins without the need for a secondary antibody. Alexa Fluor 532 is highly photostable and its absorption and emission profiles make it highly suited for use as a reporter molecule in LiquiChip assays. |
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Penta，His Biotin Conjugate consists of mouse monoclonal Penta，His Antibodies that are conjugated to biotin and bind to 6xHis-tagged proteins with high affinity and specificity. The high-affinity interaction between biotin and streptavidin allows highly sensitive detection in conjunction with Streptavidin R-PE. |
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Streptavidin-R-PE comprises streptavidin conjugated to the fluorophore R-phycoerythrin (R-PE), and binds to any biotinylated molecule (e.g., Penta，His Biotin Conjugates or other biotinylated antibodies) with high affinity. The absorption and emission profiles of R-PE are highly suited for its use as a reporter molecule in high sensitivity LiquiChip assays. |