QIAGEN RT-PCR Systems

QIAGEN offers high-quality enzymes and reagents for both one- and two-step RT-PCR. For two-step RT-PCR, Omniscript or Sensiscript RT Kits provide highly specific and sensitive reverse transcription, and HotStarTaq DNA Polymerase gives high specificity in the PCR step. Alternatively, the QIAGEN OneStep RT-PCR Kit provides all reaction components for easy and sensitive one-step RT-PCR in one tube.

Quantitative real-time two-step RT-PCR using the fluorescent dye SYBR Green can be carried out using Omniscript or Sensiscript RT Kits for reverse transcription followed by the QuantiTect SYBR Green PCR Kit for highly specific, quantitative, real-time PCR. For quantitative, real-time, one-step RT-PCR, the QuantiTect SYBR Green RT-PCR Kit can be used.

Use the table below to choose the RT-PCR system for your needs.

RT-PCR Selection Guide
RT-PCR
Primer	Application	QIAGEN enzymes
Oligo-dT or random primers	1 cDNA multiple PCR	Omniscript or Sensiscript RT HotStarTaq DNA Polymerase	2-step RT-PCR
Gene-specific primers	1 cDNA multiple PCR	Omniscript or Sensiscript RT HotStarTaq DNA Polymerase	2-step RT-PCR
Gene-specific primers	1 cDNA 1 PCR	QIAGEN OneStep RT-PCR Kit	1-step RT-PCR
Quantitative, real-time RT-PCR
Primer	Application	QIAGEN enzymes
Oligo-dT or random primers	1 cDNA multiple PCR	Omniscript or Sensiscript RT QuantiTect SYBR Green PCR Kit	2-step RT-PCR
Gene-specific primers	1 cDNA multiple PCR	Omniscript or Sensiscript RT QuantiTect SYBR Green PCR Kit	2-step RT-PCR
Gene-specific primers	1 cDNA 1 PCR	QuantiTect SYBR Green RT-PCR Kit	1-step RT-PCR