QIAGEN Transfection Reagents

QIAGEN offers four transfection reagents to satisfy your transfection needs. These reagents are based on two distinct transfection technologies — activated-dendrimer and non-liposomal–lipid technologies — and are designed for different cell types and different applications.

PolyFect, Effectene, and SuperFect Transfection Reagents are designed for efficient transfection of cells with DNA. PolyFect Reagent is specifically optimized for transfection of COS-7, NIH/3T3, HeLa, 293, and CHO cells. Effectene Reagent and SuperFect Reagent allow high transfection efficiencies in a broad range of cell lines. Effectene Transfection Reagent is also particularly effective for primary cells and sensitive cell lines. All three transfection reagents offer a fast and easy procedure, transfection in the presence of serum, and low cytotoxicity. In addition, all three reagents are available in bulk quantities and are suitable for high-throughput transfection.

TransMessenger Transfection Reagent is a non-liposomal lipid specifically designed for transfection of cells with RNA, offering an alternative approach for transfection experiments.

If you are unsure whether to use Effectene Reagent or SuperFect Reagent for your particular cell type, the Transfection Tools web site has a searchable list of cell lines and primary cells successfully transfected using QIAGEN Transfection Reagents. If your cell type is not in this list, QIAGEN offers the Transfection Reagent Selector Kit for assessment of whether Effectene Reagent or SuperFect Reagent is better for your particular cell type.
The Transfection Tools web site also has transfection protocols, cell-culture protocols, information, and literature about QIAGEN Transfection Reagents, as well as a discussion forum and online questionnaire. Visit the Transfection Tools site for answers to your transfection questions — www.qiagen.com/transfectiontools/.