A variety of innovative purification technologies developed by QIAGEN is available for automating applications in molecular biology, molecular diagnostics, and gene therapy research. Advanced high-throughput formats designed for nucleic acid purification, 6xHis-tagged protein purification, as well as reverse transcription and PCR are ideal for automation. BioRobot Kits are designed for optimal performance in automated protocols.
| Nucleic Acid Purification Options for Molecular Biology | |||
| BioRobot Kit | Source | Nucleic acid purified | Application |
| QIAwell 96 Ultra BioRobot Kit | Bacterial cultures | Plasmids and cosmids | Reliable long-range automated sequencing, high-throughput transfection |
| QIAprep 96 Turbo BioRobot Kit | Bacterial cultures | Plasmids and cosmids | Standard sequencing and cloning applications |
| R.E.A.L. Prep 96 BioRobot Kit | Bacterial cultures | Plasmids, cosmids, BACs, PACs, and P1s | High-throughput sequencing and screening |
| MagAttract 96 Miniprep Core Kit | Bacterial cultures | Plasmids | All standard sequencing applications, cloning, and screening |
| RNeasy 96 BioRobot Kit | Animal or human cells | Total and cytoplasmic RNA | High-throughput drug screening, diagnostics, quantitative RT-PCR, TaqMan and LightCycler analysis |
| QIAquick 96 PCR BioRobot Kit | Amplification reactions | PCR products | All standard sequencing applications, cloning, screening, microarray analysis |
| Nucleic Acid Purification Options for Molecular Diagnostics | |||
| BioRobot Kit | Source | Nucleic acid purified | Application |
| QIAamp 96 DNA Blood BioRobot Kit | Blood and body fluids | Genomic DNA | Molecular assays such as HLA typing, genetic disease and predisposition testing, paternity testing |
| QIAamp 96 Virus BioRobot Kit | Plasma, serum, urine, CSF, cell-culture supernatant | Viral RNA and DNA | Viral load monitoring, virus diagnostics, blood safety screening, RT-PCR, TaqMan and LightCycler analysis, NASBA, TMA, Roche AMPLICOR assays |
| QIAamp 96 DNA Swab BioRobot Kit | Buccal swabs | Genomic DNA | Forensics, database construction, genetic disease and predisposition testing, paternity testing, HLA typing |
| 6xHis-tagged Protein Purification and Assay Options | ||
| Kit | Quantities purified | Applications |
| Ni-NTA Magnetic Agarose Beads | 3¨C15 µg protein | Protein¨Cprotein interaction assays, protein¨CDNA interaction assays, diagnostic assays, drug screening, screening engineered enzymes, micro-scale purification |
| Ni-NTA Superflow 96 BioRobot Kit | Up to 150 µg protein/well | Medium-scale purification |
| Reverse Transcription and PCR Options | ||
| Kit | Use | Applications |
| Omniscript RT Kit | Standard reverse transcription using more than 50 ng RNA | cDNA synthesis, RT-PCR, quantitative RT-PCR, primer-extension analysis |
| Sensiscript RT Kit | Reverse transcription using <50 ng RNA | cDNA synthesis, RT-PCR, quantitative RT-PCR, primer-extension analysis |
| QIAGEN Taq DNA Polymerase Kits | Standard and specialized PCR applications | General PCR, RT-PCR, differential display, multiplex PCR, PCR-based DNA fingerprinting (VNTR, STR, and RAPD) |
| HotStarTaq DNA Polymerase Kits | High-specificity hot-start PCR | Reactions using complex genomic templates or cDNA (e.g., single-cell PCR), very low-copy targets, or multiple primer pairs |
| ProofStart DNA Polymerase | For high-fidelity PCR | Cloning, site-directed mutagenesis |
| QuantiTect SYBR Green PCR Kit | For quantitative PCR | Real-time quantification of DNA and cDNA using any real-time cycler |
| QuantiTect SYBR Green RT-PCR Kit | For quantitative RT-PCR | Real-time quantification of RNA using any real-time cycler |
| QIAGEN OneStep RT-PCR Kit | Fast and efficient one-step RT-PCR | cDNA synthesis, RT-PCR, quantitative RT-PCR, primer-extension analysis |
| DyeEx 96 Kit | Sequencing reaction cleanup | Dye-terminator removal from sequencing reactions in 96-well format |