GeneOp customized gene synthesis is a powerful tool for today¡¯s most advanced biotech applications. QIAGEN Operon¡¯s gene-synthesis staff has extensive training and experience in both gene design and optimization, providing the best way to get the exact sequence you need.
Repeated regions, GC content, and codon deviation may all affect the synthesis and function of a gene. Gene expression can be optimized by eliminating rare codons and utilizing optimal codons for your host or for multiple host systems. In addition, genes can be optimized by removing secondary structure, adjusting GC content, and adding or removing restriction sites.
Operon is the industry leader in gene synthesis, with the experience to identify potential problems before they occur. The GeneOp bioinformatics team analyzes each gene before synthesis and makes design recommendations to ensure that synthesis of your gene is problem-free.
After you have approved the final gene sequence, the GeneOp system is used to design oligonucleotides that minimize the incidence of direct repeats and other unwanted secondary structures that may hinder assembly or expression of the gene. After synthesis of the oligonucleotides, each duplex DNA product is carefully assembled by proprietary annealing and ligation reactions. The assembled gene is cloned, and the sequence is verified by complete bidirectional sequencing. The final product is delivered as lyophilized DNA cloned in a standard plasmid.