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尊敬的客户，为了感谢您对QIAGEN公司的一贯支持和厚爱，我们在2007年4月15日至 2007年5月30日进行Ni-NTA Superflow Cartridge 预装柱试用装免费派送活动，快来点击下载 试用装申请表 获得试用装吧！填写
试用信息反馈表，及时和我们分享您的试用信息，更有机会赢取iPod! 2007年4月15日至2007年6月30日购买该产品享受特价优惠！还等什么，赶快行动吧！！！
联系我们：
QIAGEN有限公司上海代表处
电话：021-51345678 010-82800789
传真：021-51342500 010-82800855
| QIAGEN公司拥有纯化His标签蛋白的专利产品Ni-NTA亲和层析填料。这种Ni-NTA填料具有4个镍离子螯和位点（而普通填料只有3个）。 从而能更有效防止镍离子脱落， 避免了其他填料因镍离子脱落而造成的产物回收率低，纯度低等问题。也因为这个专利，QIAGEN还为其他公司生产Ni-NTA填料。 |
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一步到位的纯化流程，操作方便
功能强大：亲和力强，结合量大，产量高
产物纯度高：纯化树脂的特异性高，洗脱条件严谨
灵活性好：可在变性/非变性条件下进行纯化
产品规格多：提供适合ng级到kg级His标签蛋白纯化的相关产品 |
Ni-NTA系列纯化试剂适用范围：
Ni-NTA系列纯化试剂参数：
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产品名称 |
Ni-NTA Magnetic Agarose Beads |
Ni-NTA Spin Columns |
Ni-NTA Agarose |
Ni-NTA Superflow |
Ni-NTA Superflow Columns |
Ni-NTA Superflow cartridges |
纯化规模 |
Micro-scale |
Micro-scale |
Small to medium |
Small to production |
Large scale |
Large scale |
结合能力 |
0.25–1 mg/ml suspension (5%) |
150 μg/spin column (@ ~20 kDa) |
Up to 20 mg/ml (@ ~20 kDa) |
Up to 20 mg/ml ( @ ~20 kDa) |
30 mg/per column (@ ~20 kDa) |
At least 20 mg/ml (@ 20 kDa) |
树脂基质 |
Cross-linked 3% agarose |
Macroporous silica |
Sepharose CL-6B |
Superflow |
Superflow |
Superflow |
树脂结构: |
Agarose beads containing magnetic particles |
Spherical, silica particles |
Cross-linked, 6% agarose |
Highly cross-linked, 6% agarose |
Highly cross-linked, 6% agarose |
Highly cross-linked, 6% agarose |
树脂颗粒大小 |
50 μm average (range: 20–70 μm) |
16–24 μm |
45–165 μm |
60–160 μm |
60–160 μm |
60–169 μm |
最大线性流速 |
n.a |
n.d |
75-150 cm/h |
3000 |
n.a |
n.a |
最大体积流速 |
n.a |
n.d |
0.5-1.0 ml/min |
20 ml/min |
n.a |
10 ml/min
40 ml/min |
最大压力 |
n.a |
n.d |
0.2psi (0.2bar) |
140psi (10bar) |
n.a |
5 bar, |
pH 稳定性(≤2h) |
3-14 |
2-8.5 |
2-14 |
2-14 |
2–14 |
2–14 |
pH 稳定性(≥2h) |
4-12 |
3-7.5 |
3-12 |
3-12 |
3-12 |
3-12 |
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| Ni-NTA Superflow Cartridges促销信息： |
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产品名称 |
产品说明 |
货号 |
促销价 |
Ni-NTA Superflow Cartridges (5 x 1 ml) |
5 cartridges pre-filled with 1 ml Ni-NTA Superflow: for automated purification of His-tagged proteins using liquid chromatography systems |
30721 |
￥977 |
Ni-NTA Superflow Cartridges (100 x 1 ml) |
100 cartridges pre-filled with 1 ml Ni-NTA Superflow: for automated purification of His-tagged proteins using liquid chromatography systems |
30725 |
￥15565 |
Ni-NTA Superflow Cartridge (1 x 5 ml) |
1 cartridge pre-filled with 5 ml Ni-NTA Superflow: for automated purification of His-tagged proteins using liquid chromatography systems |
30760 |
￥867 |
Ni-NTA Superflow Cartridges (5 x 5 ml) |
5 cartridges pre-filled with 5 ml Ni-NTA Superflow: for automated purification of His-tagged proteins using liquid chromatography systems |
30761 |
￥3383 |
Ni-NTA Superflow Cartridges (100 x 5 ml) |
100 cartridges pre-filled with 5 ml Ni-NTA Superflow: for automated purification of His-tagged proteins using liquid chromatography systems |
30765 |
￥54065 |
其他Ni-NTA产品订购信息：
产品名称 |
产品说明 |
货号 |
价格 |
Ni-NTA Magnetic Agarose Beads (2 x 1 ml) |
2 x 1 ml nickel-charged magnetic agarose beads (5% suspension) |
36111 |
￥2,525 |
Ni-NTA Magnetic Agarose Beads (6 x 1 ml) |
6 x 1 ml nickel-charged magnetic agarose beads (5% suspension) |
36113 |
￥7,100 |
Ni-NTA Spin Columns (50) |
50 Ni-NTA Spin Columns, Collection Tubes |
31014 |
￥4,750 |
Ni-NTA Agarose (25 ml) |
25 ml nickel-charged resin (max. pressure: 2.8 psi) |
30210 |
￥3,250 |
Ni-NTA Agarose (100 ml) |
100 ml nickel-charged resin (max. pressure: 2.8 psi) |
30230 |
￥11,125 |
Ni-NTA Agarose (500 ml) |
500 ml nickel-charged resin (max. pressure: 2.8 psi) |
30250 |
￥47,875 |
Ni-NTA Superflow (25 ml) |
25 ml nickel-charged resin (max. pressure: 140 psi) |
30410 |
￥4,075 |
Ni-NTA Superflow (100 ml) |
100 ml nickel-charged resin (max. pressure: 140 psi) |
30430 |
￥13,625 |
Ni-NTA Superflow (500 ml) |
500 ml nickel-charged resin (max. pressure: 140 psi) |
30450 |
￥57,500 |
Ni-NTA Superflow Columns (12 x 1.5 ml) |
For 12 6xHis-tagged protein preps: 12 polypropylene columns containing 1.5 ml Ni-NTA Superflow |
30622 |
￥4,325 |
Ni-NTA Superflow Columns (144 x 1.5 ml) |
For 144 6xHis-tagged protein preps: 12 x 12 polypropylene columns containing 1.5 ml Ni-NTA Superflow |
30624 |
￥46,725 |
Ni-NTA Fast Start Kit (6) |
For purification and detection of six 6xHis-tagged protein preps: 6 x Fast Start Columns, Penta·His Antibody, Buffers and Reagents |
30600 |
￥3,625 |
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| 除了大家熟悉的Ni-NTA 产品外，QIAGEN还拥有体内，体外蛋白表达纯化的完整产品线。QIAGEN蛋白表达纯化产品设计的理念很明确，一切围绕His-标签进行，从载体到表达，从纯化到检测，每一个步骤QIAGEN为客户想的十分周到和细致。还等什么，赶快拿起电话订购吧！ |
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产品名称 |
产品说明 |
货号 |
价格 |
N-Terminus pQE Vector Set
QIAexpress Type IV Kit |
Expression of N-terminally 6xHis-tagged proteins |
32915 |
￥7,375 |
C-Terminus pQE Vector Set
QIAexpress Type ATG Kit |
Expression of C-terminally 6xHis-tagged proteins |
32903 |
￥7,000 |
cis-Repressed pQE Vector Set |
cis-repressed expression of N-terminally 6xHis-tagged proteins |
32923 |
￥5,650 |
pQE-TriSystem Vector |
Parallel expression of C-terminally 6xHis-tagged proteins in three expression systems |
33903 |
￥4,700 |
His·Strep pQE-TriSystem Vector Set
pQE-TriSystem Strep Vector |
Parallel expression of Strep- and His·Strep-tagged proteins in three expression systems |
32942 |
￥6,250 |
TAGZyme pQE Vector Set |
Expression of 6xHis-tagged proteins whose tag can be removed using TAGZyme Enzymes |
32932 |
￥4,750 |
pQE-30 Xa Vector |
Expression of 6xHis-tagged proteins containing a Factor Xa Protease recognition site |
33203 |
￥3,950 |
pQE-100 DoubleTag Vector DNA |
Expression of proteins tagged with both 6xHis and Tag·100 |
33003 |
￥4,075 |
QIAexpress UA Cloning Kit |
Fast and efficient PCR cloning of expression constructs |
32179 |
￥3,750 |
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产品名称 |
蛋白产量 |
货号 |
价格 |
EasyXpress Protein Synthesis Mini Kit |
up to 600 μg/ml |
32502 |
￥5,950 |
EasyXpress Protein Synthesis Maxi Kit |
up to 600 μg/ml |
32506 |
￥19,750 |
EasyXpress Random Biotin Kit |
up to 600 μg/ml |
32612 |
￥8,925 |
EasyXpress Site-Specific Biotin Kit |
up to 150 μg/ml |
32602 |
￥4,050 |
EasyXpress Mega Kit |
up to 5 mg per kit |
32516 |
￥22,700 |
EasyXpress NMR Protein Synthesis kits |
up to 5 mg per kit |
32526 |
￥22,700 |
EasyXpress Insect Kit II |
20 x 50 μl reactions |
32562 |
￥7,300 |
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产量 |
纯化方式 |
产品 |
小规模纯化 |
1-50 μg |
手动 |
Ni-NTA Magnetic Agarose Beads |
自动 |
Ni-NTA Magnetic Agarose Beads |
中等规模纯化 |
50-150 μg |
手动 |
Ni-NTA Spin Columns |
自动（96孔板） |
Ni-NTA Superflow 96 Biorobot kit |
手动 |
Ni-NTA Agarose |
大规模纯化 |
100 μg至100 mg |
手动 |
Ni-NTA Superflow |
5 mg至生产规模 |
自动 |
Ni-NTA Superflow |
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树脂类型 |
树脂体积 |
培养细胞体积 |
产量 |
回收率 (%) |
纯度* |
Ni-NTA Magnetic Agarose Beads (micro-scale) |
100 μl |
1 ml |
33 μg |
~ 90% |
~ 97% |
Ni-NTA Superflow (small-scale) |
500 μl |
320 ml |
6 mg |
~ 80% |
~ 96% |
Ni-NTA Superflow (medium-scale) |
10 ml |
1.7 l |
109 mg |
~ 80% |
~ 98% |
Ni-NTA Superflow (large-scale) |
100 ml |
18 l |
2 g |
> 88% |
~ 97% |
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检测方式 |
产品 |
货号 |
价格 |
间接检测6xHis-tagged 蛋白 |
Anti·His Antibody Selector Kit |
34698 |
￥1,325 |
RGS·His Antibody |
34610 |
￥6,625 |
RGS·His Antibody, BSA-free |
34650 |
￥6,625 |
Tetra·His Antibody, BSA-free |
34670 |
￥7,525 |
Penta·His Antibody, BSA-free |
34660 |
￥7,525 |
直接检测6xHis-tagged 蛋白 |
Penta·His HRP Conjugate Kit |
34460 |
￥4,900 |
RGS·His HRP Conjugate Kit |
34450 |
￥4,325 |
Tetra·His HRP Conjugate Kit |
34470 |
￥4,900 |
Ni-NTA HRP Conjugate |
34530 |
￥4,075 |
Ni-NTA AP Conjugate |
34510 |
￥4,075 |
免疫荧光间接检测6xHis-tagged蛋白 |
Penta·His Biotin Conjugate |
34440 |
￥3,600 |
Streptavidin–R-PE |
922721 |
￥2,225 |
免疫荧光直接检测6xHis-tagged蛋白 |
Penta·His Alexa Fluor 488 Conjugate |
35310 |
￥2,275 |
Penta·His Alexa Fluor 532 Conjugate |
35330 |
￥2,275 |
Penta·His Alexa Fluor 555 Conjugate |
35350 |
￥2,275 |
Penta·His Alexa Fluor 647 Conjugate |
35370 |
￥2,275 |
6xHis-tagged 蛋白分子量标准 |
6xHis Protein Ladder |
34705 |
￥3,250 |
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应用 |
产品 |
产品说明 |
Immunoassay(ELISA, RIA) |
Ni-NTA HisSorb Strips and Plates |
Up to 0.3μg protein immobilized per well |
Interaction studies (protein-protein, protein-DNA) |
Tag.100 antibody |
Assay of proteins expressed using the pQE-100 double tag vector without need for a protein-specific antibody |
Screening |
Ni-NTA magnetic Agarose beads |
3-15 μg protein captured per well |
Drug screening interaction assays |
LiquiChip System |
Multiplex, bead-based, homogeneous assay |
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德国QIAGEN公司上海代表处
电话：021-51345678；010-82800789
传真：021-51342500；010-82800855
Email：China@qiagen.com |
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